Rabbit anti-Human FMO5 Polyclonal Antibody | anti-FMO5 antibody

FMO5, CT (FMO5, Dimethylaniline monooxygenase [N-oxide-forming] 5, Dimethylaniline oxidase 5, Hepatic flavin-containing monooxygenase 5) (Biotin)

Cat. Num. AAA6299469

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FMO5; Polyclonal Antibody; CT (FMO5; Dimethylaniline monooxygenase [N-oxide-forming] 5; Dimethylaniline oxidase 5; Hepatic flavin-containing monooxygenase 5) (Biotin); Hepatic flavin-containing monooxygenase 5; anti-FMO5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-FMO5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: FMO5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 474-502 amino acids from the C-terminal region of human FMO5.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FMO5 Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the FMO5 antibody detected the FMO5 protein (arrow).)

Related Product Information for anti-FMO5 antibody

Metabolic N-oxidation of the diet-derived amino-trimethylamine (TMA) is mediated by flavin-containing monooxygenase and is subject to an inherited FMO3 polymorphism in man resulting in a small subpopulation with reduced TMA N-oxidation capacity resulting in fish odor syndrome Trimethylaminuria. Three forms of the enzyme, FMO1 found in fetal liver, FMO2 found in adult liver, and FMO3 are encoded by genes clustered in the 1q23-q25 region. Flavin-containing monooxygenases are NADPH-dependent flavoenzymes that catalyzes the oxidation of soft nucleophilic heteroatom centers in drugs, pesticides, and xenobiotics. Alternative splicing results in multiple transcript variants.

Product Categories/Family for anti-FMO5 antibody

Antibodies; Enzymes; Oxidase

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Ross, C.J., et al. Nat. Genet. 41(12):1345-1349(2009). Wheeler, H.E., et al. PLoS Genet. 5 (10), E1000685 (2009): Zhang, J., et al. Drug Metab. Dispos. 34(1):19-26(2006). Furnes, B., et al. Drug Metab. Dispos. 31(2):187-193(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 221316672
• NCBI GeneID: 2330
• NCBI Accession #: NP_001452.2
• NCBI GenBank Nucleotide #: NM_001461.3
• UniProt Accession #: P49326; Q8IV22; B2RBG1; C9JJD1
• Molecular Weight: 52,477 Da
• NCBI Official Full Name: dimethylaniline monooxygenase
• NCBI Official Synonym Full Names: flavin containing monooxygenase 5
• NCBI Official Symbol: FMO5
• NCBI Protein Information: dimethylaniline monooxygenase [N-oxide-forming] 5; dimethylaniline monooxygenase [N-oxide-forming] 5; FMO 5; dimethylaniline oxidase 5; hepatic flavin-containing monooxygenase 5
• UniProt Protein Name: Dimethylaniline monooxygenase [N-oxide-forming] 5
• Protein Family: Dimethylaniline monooxygenase
• UniProt Gene Name: FMO5
• UniProt Synonym Gene Names: FMO 5
• UniProt Entry Name: FMO5_HUMAN

NCBI Description

Metabolic N-oxidation of the diet-derived amino-trimethylamine (TMA) is mediated by flavin-containing monooxygenase and is subject to an inherited FMO3 polymorphism in man resulting in a small subpopulation with reduced TMA N-oxidation capacity resulting in fish odor syndrome Trimethylaminuria. Three forms of the enzyme, FMO1 found in fetal liver, FMO2 found in adult liver, and FMO3 are encoded by genes clustered in the 1q23-q25 region. Flavin-containing monooxygenases are NADPH-dependent flavoenzymes that catalyzes the oxidation of soft nucleophilic heteroatom centers in drugs, pesticides, and xenobiotics. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2009]

Uniprot Description

FMO5: In contrast with other forms of FMO it does not seem to be a drug-metabolizing enzyme. Belongs to the FMO family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.13.8; Oxidoreductase; Xenobiotic Metabolism - drug metabolism - cytochrome P450

Chromosomal Location of Human Ortholog: 1q21.1

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: FAD binding; NADP binding; flavin-containing monooxygenase activity