Flt3 Ligand Cell Lysate | FLT3LG cell lysate
Mouse Flt3 Ligand HEK293 Overexpression Lysate
Gene Names
Flt3l; Ly72L; Flt3lg
Applications
Western Blot
Synonyms
Flt3 Ligand; Mouse Flt3 Ligand HEK293 Overexpression Lysate; Mouse FLT3L/Flt3 ligand HEK293 Cell Lysate (WB positive control); fms related tyrosine kinase 3 ligand; Mouse FLT3L Overexpression Lysate; Mouse Flt3lg Overexpression Lysate; Mouse Ly72L Overexpression Lysate; FLT3LG cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
403aa
Sequence
Met1-Arg188
Applicable Applications for FLT3LG cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse FLT3L (P49772-1) (Met1-Arg188) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for FLT3LG cell lysate
FLT3L, also known as flt3 ligand, is a small molecule that acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. In vivo, FLT3L also induces the mobilization of the hematopoietic progenitors and stem cells. This may help the system to kill cancer cells. Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3L controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 -positive classical DCs and their CD103 -positive tissue counterparts.
This Mouse Flt3 Ligand overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Flt3 Ligand protein from the overexpression lysate was verified.
This Mouse Flt3 Ligand overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Flt3 Ligand protein from the overexpression lysate was verified.
References
Hannum C, et al. (1994) Ligand for FLT3/FLK2 receptor tyrosine kinase regulates growth of haematopoietic stem cells and is encoded by variant RNAs. Nature 368 (6472): 643-8.Lyman SD, et al. (1995) Identification of soluble and membrane-bound isoforms of the murine flt3 ligand generated by alternative splicing of mRNAs. Oncogene 10 (1): 149-57.Lyman SD, et al. (1994) Molecular cloning of a ligand for the flt3/flk-2 tyrosine kinase receptor: a proliferative factor for primitive hematopoietic cells. Cell 75 (6): 1157-67.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,259 Da
NCBI Official Full Name
fms-related tyrosine kinase 3 ligand
NCBI Official Synonym Full Names
FMS-like tyrosine kinase 3 ligand
NCBI Official Symbol
Flt3l
NCBI Official Synonym Symbols
Ly72L; Flt3lg
NCBI Protein Information
fms-related tyrosine kinase 3 ligand
UniProt Protein Name
Fms-related tyrosine kinase 3 ligand
Protein Family
UniProt Gene Name
Flt3lg
UniProt Synonym Gene Names
Flt3l; Flt3 ligand; Flt3L
UniProt Entry Name
FLT3L_MOUSE
Uniprot Description
FLT3LG: Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytokine; Ligand, receptor tyrosine kinase; Membrane protein, integral
Cellular Component: cell surface; extracellular space
Molecular Function: protein homodimerization activity; receptor tyrosine kinase binding
Biological Process: embryonic hemopoiesis; homeostasis of number of cells within a tissue; lymphocyte differentiation; positive regulation of cell cycle; positive regulation of cell proliferation; positive regulation of myoblast differentiation; positive regulation of natural killer cell differentiation; positive regulation of protein amino acid phosphorylation; positive regulation of transcription from RNA polymerase II promoter; regulation of myeloid dendritic cell activation
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Product Notes
The FLT3LG flt3lg (Catalog #AAA8117056) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 403aa. AAA Biotech's Flt3 Ligand can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the FLT3LG flt3lg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Arg18 8. It is sometimes possible for the material contained within the vial of "Flt3 Ligand, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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