JAM-A/Junctional Adhesion Molecule A Cell Lysate | F11R cell lysate
Mouse JAM-A/Junctional Adhesion Molecule A HEK293 Overexpression Lysate
Gene Names
F11r; JAM; Jcam; JAM-1; JAM-A; Jcam1; Ly106; ESTM33; AA638916; 9130004G24
Applications
Western Blot
Synonyms
JAM-A/Junctional Adhesion Molecule A; Mouse JAM-A/Junctional Adhesion Molecule A HEK293 Overexpression Lysate; Mouse JAM-A/F11R HEK293 Cell Lysate (WB positive control); F11 receptor; Mouse 9130004G24 Overexpression Lysate; Mouse AA638916 Overexpression Lysate; Mouse ESTM33 Overexpression Lysate; Mouse JAM Overexpression Lysate; Mouse JAM-1 Overexpression Lysate; Mouse JAM-A Overexpression Lysate; Mouse Jcam Overexpression Lysate; Mouse Jcam1 Overexpression Lysate; Mouse Ly106 Overexpression Lysate; F11R cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
457aa
Sequence
Met1-Ala242
Applicable Applications for F11R cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse F11R (NP_766235.1) extracellular domain (Met 1-Ala 242) was fused with the Fc region of human IgG1 at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for F11R cell lysate
Junctional adhesion molecule-A (JAM-A), also known as F11 receptor (F11R) or Cluster of Differentiation 321 (CD321), is a transmembrane protein expressed at tight junctions of epithelial and endothelial cells, as well as on circulating leukocytes. JAM-A protein serves as a serotype-independent receptor for mammalian orthoreoviruses (reoviruses). It is also a ligand for the integrin LFA1, involves in leukocyte transmigration. As a cell adhesion molecule of the immunoglobulin superfamily, JAM-A protein involves in platelet adhesion, secretion and aggregation, and plays a crucial role in inflammatory thrombosis and atherosclerosis. In addition, it may be a potential therapeutic target for breast cancer.
This Mouse JAM-A/Junctional Adhesion Molecule A overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of JAM-A/Junctional Adhesion Molecule A protein from the overexpression lysate was verified.
This Mouse JAM-A/Junctional Adhesion Molecule A overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of JAM-A/Junctional Adhesion Molecule A protein from the overexpression lysate was verified.
References
Guglielmi KM, et al. (2007) Reovirus binding determinants in junctional adhesion molecule-A. J Biol Chem. 282(24): 17930-40.Yeung D, et al. (2008) Decreased junctional adhesion molecule-A expression during blood-brain barrier breakdown. Acta Neuropathol. 115(6): 635-42.Ong KL, et al. (2009) Elevated plasma level of soluble F11 receptor/junctional adhesion molecule-A (F11R/JAM-A) in hypertension. Am J Hypertens. 22(5): 500-5.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,424 Da
NCBI Official Full Name
junctional adhesion molecule A
NCBI Official Synonym Full Names
F11 receptor
NCBI Official Symbol
F11r
NCBI Official Synonym Symbols
JAM; Jcam; JAM-1; JAM-A; Jcam1; Ly106; ESTM33; AA638916; 9130004G24
NCBI Protein Information
junctional adhesion molecule A; BV11 antigen; F11 receptor/F11R; junction cell adhesion molecule A; junction cell adhesion molecule1; junctional adhesion molecule 1
UniProt Protein Name
Junctional adhesion molecule A
Protein Family
UniProt Gene Name
F11r
UniProt Synonym Gene Names
Jam1; Jcam; Jcam1; JAM-A; JAM-1
UniProt Entry Name
JAM1_MOUSE
Uniprot Description
JAM-A: Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly. Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus. Belongs to the immunoglobulin superfamily.
Protein type: Membrane protein, integral; Cell adhesion
Cellular Component: microtubule cytoskeleton; tight junction; membrane; integral to membrane; plasma membrane; intercellular junction; cytoplasmic vesicle; cell junction
Molecular Function: protein binding; PDZ domain binding
Biological Process: viral reproduction; epithelial cell differentiation; actomyosin structure organization and biogenesis; cell adhesion; positive regulation of blood pressure; intestinal absorption
