Ephrin A5 Active Protein | EFNA5 active protein

Ephrin A5 Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8122858

• Gene Names: Efna5; AL-1; Epl7; RAGS; EFL-5; LERK-7; AV158822; Ephrin-A5
• Purity: >85% as determined by SDS-PAGE
• Synonyms: Ephrin A5; Ephrin A5 Protein; Mouse; Recombinant (hFc Tag); Mouse Ephrin-A5/EFNA5 Protein (Fc Tag); AL-1 Protein; AV158822 Protein; EFL-5 Protein; Ephrin-A5 Protein; Epl7 Protein; LERK-7 Protein; RAGS Protein; ephrin-A5; EFNA5 active protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asn203

• Species: Mouse
• Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse EPHA4 at 2ug/ml (100uL/well) can bind mouse EFNA5-Fc with a linear range of 1.28-32ng/mL.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 21
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the mouse EFNA5 isoform long (O08543-1) without the pro peptide (Met 1-Asn 203) was as fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for EFNA5 active protein

Background: Ephrin-A5 also known as EFNA5, is a member of the Ephrin family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. Ephrin-A5/EFNA5 may function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Ephrin-A5/EFNA5 also serves as a cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion.

References

Frisa©n J, et al. (1998) Ephrin-A5 (AL-1/RAGS) is essential for proper retinal axon guidance and topographic mapping in the mammalian visual system. Neuron. 20(2): 235-43.Feldheim DA, et al. (2000) Genetic analysis of ephrin-A2 and ephrin-A5 shows their requirement in multiple aspects of retinocollicular mapping. Neuron. 25(3): 563-74.Wahl S, et al. (2000) Ephrin-A5 induces collapse of growth cones by activating Rho and Rho kinase. J Cell Biol. 149(2): 263-70.

NCBI and Uniprot Product Information

• NCBI GI #: 6753724
• NCBI GeneID: 13640
• NCBI Accession #: NP_034239.1
• NCBI GenBank Nucleotide #: NM_010109.3
• UniProt Accession #: O08543; O08544
• Molecular Weight: 26,339 Da
• NCBI Official Full Name: ephrin-A5 isoform 2
• NCBI Official Synonym Full Names: ephrin A5
• NCBI Official Symbol: Efna5
• NCBI Official Synonym Symbols: AL-1; Epl7; RAGS; EFL-5; LERK-7; AV158822; Ephrin-A5
• NCBI Protein Information: ephrin-A5; EPH-related receptor tyrosine kinase ligand 7
• UniProt Protein Name: Ephrin-A5
• Protein Family: Ephrin
• UniProt Gene Name: Efna5
• UniProt Synonym Gene Names: Epl7; Eplg7; Lerk7; LERK-7
• UniProt Entry Name: EFNA5_MOUSE