VSIG4 cell lysate
Mouse VSIG4 HEK293 Overexpression Lysate
Gene Names
Vsig4; CRIg; Z39IG; BC025105; A530061A11
Applications
Western Blot
Synonyms
VSIG4; Mouse VSIG4 HEK293 Overexpression Lysate; Mouse VSIG4 HEK293 Cell Lysate (WB positive control); V-set and immunoglobulin domain containing 4; Mouse A530061A11 Overexpression Lysate; Mouse BC025105 Overexpression Lysate; Mouse CRIg Overexpression Lysate; Mouse Z39IG Overexpression Lysate; VSIG4 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
179aa
Sequence
Met1-Pro187
Applicable Applications for VSIG4 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the extracellular domain (Met 1-Pro 187) of mouse VSIG4 (NP_808457.1) precursor was expressed with a C-terminal polyhistidine tag.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for VSIG4 cell lysate
VSIG4 (V-set and immunoglobulin domain containing 4), also known as complement receptor of the immunoglobulin superfamily (CRIg) and Z39Ig, is a type I transmembrane glycoprotein. It is a B7 family-related protein and an Ig superfamily member. In contrast to the B7 family members which contain two IgG domains, VSIG4 contains one complete V-type I g domain and a truncated C-type I g domain. VSIG4 is exclusively expressed on tissue resident macrophages and binds to multimers of C3b and iC3b that are covalently attached to particle surfaces. No VSIG4 expression appears to be present in T and B cells. VSIG4 functions as a negative regulator of T cell activation, and may be involved in the maintenance of peripheral T cell tolerance, and is also identified as a potent suppressor of established inflammation. Mouse VSIG4 is synthesized as a 28 amino acid precursor that contains a signal sequence, a V-type I g domain (aa 36-115), one potential N-linked glycosylation site, and a single transmembrane domain. The V-type I g domain of mouse VSIG4 shares 86% and 8% aa sequence identity with the V-type I g domains of rat and human VSIG4, respectively.
This Mouse VSIG4 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of VSIG4 protein from the overexpression lysate was verified.
This Mouse VSIG4 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of VSIG4 protein from the overexpression lysate was verified.
References
Vogt, L. et al., 2006, J Clin Invest.116: 2817-2826. Helmy, K. et al., 2006, Cell. 124:915-927. Wiesmann, C. et al., 2006, Nature. 444:217-220. Zang, X. et al., 2006, J Clin Invest. 116: 2590-2593. Katschke, KJ. et al., 2007, J. Exp. Med. 204:1319-1325. He, JQ. et al., 2008, Mol Immunol. 45: 4041-4047.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
V-set and immunoglobulin domain-containing protein 4
NCBI Official Synonym Full Names
V-set and immunoglobulin domain containing 4
NCBI Official Symbol
Vsig4
NCBI Official Synonym Symbols
CRIg; Z39IG; BC025105; A530061A11
NCBI Protein Information
V-set and immunoglobulin domain-containing protein 4
Protein Family
Research Articles on VSIG4
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Product Notes
The VSIG4 (Catalog #AAA8116448) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 179aa. AAA Biotech's VSIG4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the VSIG4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Pro18 7. It is sometimes possible for the material contained within the vial of "VSIG4, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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