TMED4 cell lysate
Human TMED4 HEK293 Overexpression Lysate
Gene Names
TMED4; HNLF; ERS25; p24a3; GMP25iso; p24alpha3
Applications
Western Blot
Synonyms
TMED4; Human TMED4 HEK293 Overexpression Lysate; Human TMED4/ERS25 HEK293 Cell Lysate (WB positive control); transmembrane emp24 protein transport domain containing 4; Human ERS25 Overexpression Lysate; Human GMP25iso Overexpression Lysate; Human HNLF Overexpression Lysate; Human p24alpha3 Overexpression Lysate; TMED4 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
399aa
Sequence
Met1-Arg194
Applicable Applications for TMED4 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human TMED4 (NP_872353.2) (Met1-Arg194) was expressed with the Fc region of mouse IgG1 at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for TMED4 cell lysate
TMED4, also known as ERS25, belongs to the EMP24/GP25L family. TMED4 may play a role in the regulation of heat-shock response and apoptosis. It is involved in vesicular protein trafficking, mainly in the early secretory pathway. TMED4 may also play a role in the biosynthesis of secreted cargo including processing. It functions in the regulation of heat-shock response and apoptosis. TMED4 also is involved in endoplasmic reticulum stress response.
This Human TMED4 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of TMED4 protein from the overexpression lysate was verified.
This Human TMED4 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of TMED4 protein from the overexpression lysate was verified.
References
Hartley JL. et al., 2001, Genome Res. 10 (11): 1788-95. Matoba R. et al., 1994, Gene. 146 (2): 199-207. Matsuda A. et al., 2003, Oncogene. 22 (21): 3307-18.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,070 Da
NCBI Official Full Name
transmembrane emp24 domain-containing protein 4 isoform 1
NCBI Official Synonym Full Names
transmembrane p24 trafficking protein 4
NCBI Official Symbol
TMED4
NCBI Official Synonym Symbols
HNLF; ERS25; p24a3; GMP25iso; p24alpha3
NCBI Protein Information
transmembrane emp24 domain-containing protein 4
UniProt Protein Name
Transmembrane emp24 domain-containing protein 4
Protein Family
UniProt Gene Name
TMED4
UniProt Synonym Gene Names
ERS25; ERS25; p24alpha3
Uniprot Description
Involved in vesicular protein trafficking, mainly in the early secretory pathway. targeting. Involved in the maintenance of the Golgi apparatus. Appears to play a role in the biosynthesis of secreted cargo including processing. Involved in endoplasmic reticulum stress response. May play a role in the regulation of heat-shock response and apoptosis ().
Research Articles on TMED4
Similar Products
Product Notes
The TMED4 tmed4 (Catalog #AAA8115253) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 399aa. AAA Biotech's TMED4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the TMED4 tmed4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Arg19 4. It is sometimes possible for the material contained within the vial of "TMED4, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
