PRSS3 cell lysate
Human PRSS3 HEK293 Overexpression Lysate
Gene Names
PRSS3; T9; MTG; TRY3; TRY4; PRSS4
Applications
Western Blot
Synonyms
PRSS3; Human PRSS3 HEK293 Overexpression Lysate; Human Trypsin-3/PRSS3 HEK293 Cell Lysate (WB positive control); protease; serine 3; Human MTG Overexpression Lysate; Human PRSS4 Overexpression Lysate; Human RP11-176F3.3 Overexpression Lysate; Human T9 Overexpression Lysate; Human TRY3 Overexpression Lysate; Human TRY4 Overexpression Lysate; PRSS3 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
243aa
Sequence
Met1-Ser247
Applicable Applications for PRSS3 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human PRSS3 isoform c (P35030-3) (Met 1-Ser 247) was expressed, with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for PRSS3 cell lysate
Trypsin-3, also known as Trypsin III, brain trypsinogen, Serine protease 3 and PRSS3, is a secreted protein that belongs to the peptidase S1 family. Trypsin-3/PRSS3 is expressed is in pancreas and brain. It contains one peptidase S1 domain. Trypsin-3/PRSS3 can degrade intrapancreatic trypsin inhibitors that protect against CP. Genetic variants that cause higher mesotrypsin activity might increase the risk for chronic pancreatitis (CP). A sustained imbalance of pancreatic proteases and their inhibitors seems to be important for the development of CP. The trypsin inhibitor-degrading activity qualified PRSS3 as a candidate for a novel CP susceptibility gene. Trypsin-3/PRSS3 has been implicated as a putative tumor suppressor gene due to its loss of expression, which is correlated with promoter hypermethylation, in esophageal squamous cell carcinoma and gastric adenocarcinoma.
This Human PRSS3 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of PRSS3 protein from the overexpression lysate was verified.
This Human PRSS3 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of PRSS3 protein from the overexpression lysate was verified.
Product Categories/Family for PRSS3 cell lysate
References
Venter JC. et al., 2001, Science 291:1304-51. Marsit, CJ. et al., 2005, Mol Carcinog 44 (2):146-50. Rowen, L. et al., 2005, Mol Biol Evol. 22 (8):1712-20. Rosendahl, J. et al., 2010, Pancreatology. 10 (2-3):243-9
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,924 Da
NCBI Official Full Name
trypsin-3 isoform 3 preproprotein
NCBI Official Synonym Full Names
protease, serine 3
NCBI Official Symbol
PRSS3
NCBI Official Synonym Symbols
T9; MTG; TRY3; TRY4; PRSS4
NCBI Protein Information
trypsin-3
UniProt Protein Name
Trypsin-3
UniProt Gene Name
PRSS3
UniProt Synonym Gene Names
PRSS4; TRY3; TRY4
UniProt Entry Name
TRY3_HUMAN
Uniprot Description
Digestive protease specialized for the degradation of trypsin inhibitors. In the ileum, may be involved in defensin processing, including DEFA5.
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Product Notes
The PRSS3 prss3 (Catalog #AAA8114808) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 243aa. AAA Biotech's PRSS3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the PRSS3 prss3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Ser24 7. It is sometimes possible for the material contained within the vial of "PRSS3, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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