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IL17RB cell lysate

Mouse IL17RB HEK293 Overexpression Lysate

Gene Names
Il17rb; Evi27; Il17br; IL-17ER; IL17RH1; IL-17Rh1
Applications
Western Blot
Synonyms
IL17RB; Mouse IL17RB HEK293 Overexpression Lysate; Mouse IL17BR/IL17RB/IL-17 Receptor B HEK293 Cell Lysate (WB positive control); interleukin 17 receptor B; Mouse Evi27 Overexpression Lysate; Mouse IL-17ER Overexpression Lysate; Mouse IL-17Rh1 Overexpression Lysate; Mouse Il17br Overexpression Lysate; Mouse IL17RH1 Overexpression Lysate; IL17RB cell lysate
Ordering
For Research Use Only!
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
510aa
Sequence
Met1-Gly286
Applicable Applications for IL17RB cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse Il17rb (NP_062529.2) (Met1-Gly286) was expressed with the Fc region of human IgG1 at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for IL17RB cell lysate
IL17RB (Interleukin 17 Receptor B) is a Protein Coding gene. IL17RB is the receptor for IL17E, the only member of the IL17 family promoting Th2 reactions. IL17RB is induced on human macrophages by IL4 and enhanced by TGFbeta. Overexpression of IL17RB is associated with poor prognosis and the short survival of breast cancer patients.IL17RB/IL17B signaling triggers a substantial increase in cell growth, proliferation, and migration through the activation of NF-kappaB as well as the up-regulation of the Bcl-2. IL17RB may be the only gene expressed in CD4+ T cells whose transcript measurement is correlated with the variation in IgE level in asthmatics. Diseases associated with IL17RB include Chronic Mucocutaneous Candidiasis and Seborrheic Infantile Dermatitis.

This Mouse IL17RB overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of IL17RB protein from the overexpression lysate was verified.
Product Categories/Family for IL17RB cell lysate
References
Rickel EA, et al.. (2008) Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities. J Immunol. 181(6): 4299-310.Stock P, et al.. (2009) Induction of airway hyperreactivity by IL-25 is dependent on a subset of invariant NKT cells expressing IL-17RB. J Immunol. 182(8): 5116-22.Wang H, et al.. (2010) Allergen challenge of peripheral blood mononuclear cells from patients with seasonal allergic rhinitis increases IL-17RB, which regulates basophil apoptosis and degranulation. Clin Exp Allergy. 40(8): 1194-202.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
55,617 Da
NCBI Official Full Name
interleukin-17 receptor B
NCBI Official Synonym Full Names
interleukin 17 receptor B
NCBI Official Symbol
Il17rb
NCBI Official Synonym Symbols
Evi27; Il17br; IL-17ER; IL17RH1; IL-17Rh1
NCBI Protein Information
interleukin-17 receptor B; IL-17RB; IL-17B receptor; IL-17 receptor B; IL-17 receptor homolog 1; interleukin 17B receptor; interleukin-17B receptor; ecotropic viral integration site 27
UniProt Protein Name
Interleukin-17 receptor B
Protein Family
UniProt Gene Name
Il17rb
UniProt Synonym Gene Names
Evi27; Il17br; IL-17 receptor B; IL-17RB; IL-17ER; IL-17Rh1; IL17Rh1; IL-17B receptor

Uniprot Description

Receptor for the proinflammatory cytokines IL17B and IL17E. May play a role in controlling the growth and/or differentiation of hematopoietic cells.

Research Articles on IL17RB

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Product Notes

The IL17RB il17rb (Catalog #AAA8117049) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 510aa. AAA Biotech's IL17RB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the IL17RB il17rb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Gly28 6. It is sometimes possible for the material contained within the vial of "IL17RB, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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