IL13Ra1 cell lysate
Human IL13RA1 HEK293 Overexpression Lysate
Gene Names
IL13RA1; NR4; CD213A1; IL-13Ra
Applications
Western Blot
Synonyms
IL13RA1; Human IL13RA1 HEK293 Overexpression Lysate; Human IL13RA1 HEK293 Cell Lysate (WB positive control); interleukin 13 receptor; alpha 1; Human CD213A1 Overexpression Lysate; Human IL-13Ra Overexpression Lysate; Human NR4 Overexpression Lysate; IL13Ra1 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
333aa
Sequence
Met1-Thr343
Applicable Applications for IL13Ra1 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human IL13Ralpha1 (NP_001551.1) extracellular domain (Met 1-Thr 343) expressed, with a C-terminal polyhistidine tag.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for IL13Ra1 cell lysate
Interleukin 13 receptor, alpha 1, also known as IL13RA1/IL-13RA1 and CD213A1 (cluster of differentiation 213A1), is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. IL13RA1/IL-13RA1 serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2 and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3, and STAT6 induced by IL13 and IL4. IL13RA1/IL-13RA1 binds with low affinity to interleukin-13 (IL13). This subunit together with IL4RA can form a functional receptor for IL13. IL13RA1/IL-13RA1 also serves as an alternate accessory protein to the common cytokine receptor gamma chain for interleukin-4 (IL4) signaling, but cannot replace the function of IL2RG in allowing enhanced interleukin-2 (IL2) binding activity.
This Human IL13RA1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of IL13RA1 protein from the overexpression lysate was verified.
This Human IL13RA1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of IL13RA1 protein from the overexpression lysate was verified.
Product Categories/Family for IL13Ra1 cell lysate
References
Kawakami M, et al. (2002) Mutation and functional analysis of IL-13 receptors in human malignant glioma cells. Oncol Res. 12 (11-12): 459-67.Umeshita-Suyama R, et al. (2000) Characterization of IL-4 and IL-13 signals dependent on the human IL-13 receptor alpha chain 1: redundancy of requirement of tyrosine residue for STAT3 activation. Int Immunol. 12 (11): 1499-509.He JQ, et al. (2003) Polymorphisms in the IL13, IL13RA1, and IL4RA genes and rate of decline in lung function in smokers. Am J Respir. Cell Mol Biol. 28 (3): 379-85.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,760 Da
NCBI Official Full Name
interleukin-13 receptor subunit alpha-1
NCBI Official Synonym Full Names
interleukin 13 receptor, alpha 1
NCBI Official Symbol
IL13RA1
NCBI Official Synonym Symbols
NR4; CD213A1; IL-13Ra
NCBI Protein Information
interleukin-13 receptor subunit alpha-1; CT19; IL-13RA1; IL-13R-alpha-1; CD213a1 antigen; IL-13R subunit alpha-1; cancer/testis antigen 19; IL13 receptor alpha-1 chain; IL-13 receptor subunit alpha-1; bB128O4.2.1 (interleukin 13 receptor, alpha 1)
UniProt Protein Name
Interleukin-13 receptor subunit alpha-1
Protein Family
UniProt Gene Name
IL13RA1
UniProt Synonym Gene Names
IL13R; IL13RA; IL-13 receptor subunit alpha-1; IL-13R subunit alpha-1; IL-13R-alpha-1; IL-13RA1; CT19
UniProt Entry Name
I13R1_HUMAN
NCBI Description
The protein encoded by this gene is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. This subunit serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4. [provided by RefSeq, Jul 2008]
Uniprot Description
IL13R: Binds with low affinity to interleukin-13 (IL13). Together with IL4RA can form a functional receptor for IL13. Also serves as an alternate accessory protein to the common cytokine receptor gamma chain for interleukin-4 (IL4) signaling, but cannot replace the function of IL2RG in allowing enhanced interleukin-2 (IL2) binding activity. Belongs to the type I cytokine receptor family. Type 5 subfamily.
Protein type: Receptor, cytokine; Membrane protein, integral
Chromosomal Location of Human Ortholog: Xq24
Cellular Component: interleukin-13 receptor complex; plasma membrane
Molecular Function: protein binding; interleukin-13 receptor activity
Biological Process: positive regulation of immunoglobulin production; cell surface receptor linked signal transduction; positive regulation of B cell proliferation
Research Articles on IL13Ra1
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Product Notes
The IL13Ra1 il13ra1 (Catalog #AAA8114246) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 333aa. AAA Biotech's IL13RA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the IL13Ra1 il13ra1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Thr34 3. It is sometimes possible for the material contained within the vial of "IL13RA1, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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