GPHA2 cell lysate
Human GPHA2 HEK293 Overexpression Lysate
Gene Names
GPHA2; A2; GPA2; ZSIG51
Applications
Western Blot
Synonyms
GPHA2; Human GPHA2 HEK293 Overexpression Lysate; Human GPHA2/Glycoprotein hormone alpha 2 HEK293 Cell Lysate (WB positive control); glycoprotein hormone alpha 2; Human A2 Overexpression Lysate; Human GPA2 Overexpression Lysate; Human ZSIG51 Overexpression Lysate; GPHA2 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
117aa
Sequence
Met1-Tyr129
Applicable Applications for GPHA2 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human GPHA2 (Q96T91) (Met1-Tyr129) was expressed with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for GPHA2 cell lysate
GPHA2 is a member of the glycoprotein hormones subunit alpha family. Glycoprotein hormones consist of two subunits, the common alpha-and specific beta-subunits, which associate noncovalently to form a heterodimer. The alpha-subunit combines with four distinct beta-subunits giving rise to four biologically active hormones in the human: FSH, LH, TSH, and CG. GPHA2 and glycoprotein hormone beta 5 (GPHB5) can form a noncovalent heterodimer. GPHA2 can be detected in a variety of tissues. Recombinant A2/B5 heterodimeric glycoproteins activate human TSH receptors, but not LH and FSH receptors, and shows high affinity to TSH receptors in a radioligand receptor assay. The heterodimer also stimulates cAMP production and thymidine incorporation by cultured thyroid cells and increases serum thyroxine levels in TSH-suppressed rats in vivo. This new heterodimeric glycoprotein hormone was named Thyrostimulin based on its thyroid-stimulating activity. The expression of Thyrostimulin in the anterior pituitary known to express TSH receptors suggested a paracrine mechanism.
This Human GPHA2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of GPHA2 protein from the overexpression lysate was verified.
This Human GPHA2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of GPHA2 protein from the overexpression lysate was verified.
References
Hsu SY. et al., 2002, Science. 295 (5555): 671-4. Suzuki C. et al., 2007, Regul Pept. 142 (1-2): 60-7. Breous E. et al., 2006, Mol Cell Endocrinol. 245 (1-2): 169-80.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14,163 Da
NCBI Official Full Name
glycoprotein hormone alpha-2
NCBI Official Synonym Full Names
glycoprotein hormone alpha 2
NCBI Official Symbol
GPHA2
NCBI Official Synonym Symbols
A2; GPA2; ZSIG51
NCBI Protein Information
glycoprotein hormone alpha-2; cysteine knot protein; glycoprotein alpha 2; putative secreted protein Zsig51; thyrostimulin subunit alpha
UniProt Protein Name
Glycoprotein hormone alpha-2
Protein Family
UniProt Gene Name
GPHA2
UniProt Synonym Gene Names
GPA2; ZSIG51
UniProt Entry Name
GPHA2_HUMAN
NCBI Description
GPHA2 is a cystine knot-forming polypeptide and a subunit of the dimeric glycoprotein hormone family (Hsu et al., 2002 [PubMed 12089349]).[supplied by OMIM, Mar 2008]
Uniprot Description
GPHA2: Stimulates the thyroid. Binds and activates THSR, leads to increased cAMP production. Belongs to the glycoprotein hormones subunit alpha family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 11q13.1
Cellular Component: extracellular region
Molecular Function: hormone activity
Research Articles on GPHA2
Similar Products
Product Notes
The GPHA2 gpha2 (Catalog #AAA8115296) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 117aa. AAA Biotech's GPHA2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the GPHA2 gpha2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Tyr12 9. It is sometimes possible for the material contained within the vial of "GPHA2, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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