DCBLD2 cell lysate
Human DCBLD2 HEK293 Overexpression Lysate
Gene Names
DCBLD2; ESDN; CLCP1
Applications
Western Blot
Synonyms
DCBLD2; Human DCBLD2 HEK293 Overexpression Lysate; Human DCBLD2/ESDN/CLCP1 HEK293 Cell Lysate (WB positive control); discoidin; CUB and LCCL domain containing 2; Human CLCP1 Overexpression Lysate; Human ESDN Overexpression Lysate; DCBLD2 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
473aa
Sequence
Met1-Ala482
Applicable Applications for DCBLD2 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human DCBLD2 (NP_563615.3) (Met1-Ala482) was expressed with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for DCBLD2 cell lysate
DCBLD2, also known as ESDN and CLCP1, localizes in various compartments. DCBLD2 is up-regulated in lung cancers and is regulated by transcription factor AP-2 alpha (TFAP2A), a component of activator protein-2 (AP-2) that is known to regulate IL-8 production in human lung fibroblasts and epithelial cells. DCBLD2 could be related to FEV(1)-related phenotypes in asthmatics. DCBLD2 gene is expressed at very high level. DCBLD2 is proposed to participate in processes such as intracellular receptor mediated signaling pathway, negative regulation of cell growth and so on.
This Human DCBLD2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of DCBLD2 protein from the overexpression lysate was verified.
This Human DCBLD2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of DCBLD2 protein from the overexpression lysate was verified.
References
Kobuke K. et al., 2001, J Biol Chem. 276 (36): 34105-14. Adeghi MM. et al., 2007, Am J Transplant. 7 (9): 2098-105. Chen Y. et al., 2007, Proteomics. 7 (14): 2384-97. Park TJ. et al., 2012, J Korean Med Sci. 27 (4): 343-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
86,657 Da
NCBI Official Full Name
discoidin, CUB and LCCL domain-containing protein 2
NCBI Official Synonym Full Names
discoidin, CUB and LCCL domain containing 2
NCBI Official Symbol
DCBLD2
NCBI Official Synonym Symbols
ESDN; CLCP1
NCBI Protein Information
discoidin, CUB and LCCL domain-containing protein 2; 1700055P21Rik; coagulation factor V/VIII-homology domains protein 1; CUB, LCCL and coagulation factor V/VIII-homology domains protein 1; endothelial and smooth muscle cell-derived neuropilin-like protei
UniProt Protein Name
Discoidin, CUB and LCCL domain-containing protein 2
Protein Family
UniProt Gene Name
DCBLD2
UniProt Synonym Gene Names
CLCP1; ESDN
UniProt Entry Name
DCBD2_HUMAN
Uniprot Description
DCBLD2: a type-I transmembrane protein. May be involved in vascular remodeling and influence vascular smooth muscle cell proliferation. Contains a CUB domain and a coagulation factor V/VIII homology domain, similar to the structure of the neuropilins. Plays a role in cell motility. SEMA4B appears to be one of its ligands. Strongly expressed in nerve bundles. Highly expressed in testis, heart, skeletal muscle and also in cultured vascular smooth muscle cells. Increased in lung cancers during the process of tumor progression. A target of EGF signaling in the A431 cervical cancer cell-line. Two isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Cell adhesion
Chromosomal Location of Human Ortholog: 3q12.1|3
Cellular Component: cell surface; integral to plasma membrane
Molecular Function: protein binding
Biological Process: intracellular receptor-mediated signaling pathway; wound healing; negative regulation of cell growth
Research Articles on DCBLD2
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Product Notes
The DCBLD2 dcbld2 (Catalog #AAA8115217) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 473aa. AAA Biotech's DCBLD2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the DCBLD2 dcbld2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Ala48 2. It is sometimes possible for the material contained within the vial of "DCBLD2, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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