CD82 cell lysate

Human CD82 HEK293 Overexpression Lysate

Cat. Num. AAA8114943

• Gene Names: CD82; R2; 4F9; C33; IA4; ST6; GR15; KAI1; SAR2; TSPAN27
• Applications: Western Blot
• Synonyms: CD82; Human CD82 HEK293 Overexpression Lysate; Human CD82/KAI-1 HEK293 Cell Lysate (WB positive control); CD82 molecule; Human 4F9 Overexpression Lysate; Human C33 Overexpression Lysate; Human GR15 Overexpression Lysate; Human IA4 Overexpression Lysate; Human KAI-1 Overexpression Lysate; Human KAI1 Overexpression Lysate; Human R2 Overexpression Lysate; Human SAR2 Overexpression Lysate; Human ST6 Overexpression Lysate; Human TSPAN27 Overexpression Lysate; CD82 cell lysate

• Host: HEK293 Cells
• Form/Format: 1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).; Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
• Sequence Positions: 129aa
• Sequence: Gly111-Leu228

Applicable Applications for CD82 cell lysate

Western Blot (WB)

Application Notes

WB: Use at an assay dependent dilution.

Species

Human

Recommend Usage

1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.

Sequence Construction

A DNA sequence encoding the second extracellular domain of human CD82 (P27701-1) (Gly 111-Leu 228) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.

Preparation Method

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.

Preparation and Storage

Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.