CD1D cell lysate
Mouse CD1D HEK293 Overexpression Lysate
Gene Names
Cd1d1; Cd1a; Cd1d; CD1.1; Ly-38; AI747460
Applications
Western Blot
Synonyms
CD1D; Mouse CD1D HEK293 Overexpression Lysate; Mouse CD1D/R3G1 HEK293 Cell Lysate (WB positive control); CD1d molecule; Mouse AI747460 Overexpression Lysate; Mouse CD1.1 Overexpression Lysate; Mouse Cd1a Overexpression Lysate; Mouse Cd1d Overexpression Lysate; Mouse Ly-38 Overexpression Lysate; CD1D cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
532aa
Sequence
Met1-Gly305
Applicable Applications for CD1D cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the extracellular domain of mouse CD1D (NP_031665.2) (Met 1-Gly 305) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for CD1D cell lysate
The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 1 (CD1) is a member of the CD system. It's a family of glycoproteins expressed on the surface of various human antigen-presenting cells which are implicated in the presentation of lipid antigens to T-cells. Due to the different lipid anchoring, the CD1 family is classified into two groups: group1 (CD1a-c) and group2 (CD1d). CD1d with lipid antigens activate NK T-cells which rapidly produce Th1 and Th2 cytokines after been activated.
This Mouse CD1D overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CD1D protein from the overexpression lysate was verified.
This Mouse CD1D overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CD1D protein from the overexpression lysate was verified.
References
Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.Joyce S. (2001) CD1d and natural T cells: how their properties jump-start the immune system. Cell Mol Life Sci. 58 (3): 442-69.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,554 Da
NCBI Official Full Name
antigen-presenting glycoprotein CD1d1
NCBI Official Synonym Full Names
CD1d1 antigen
NCBI Official Symbol
Cd1d1
NCBI Official Synonym Symbols
Cd1a; Cd1d; CD1.1; Ly-38; AI747460
NCBI Protein Information
antigen-presenting glycoprotein CD1d1; T-cell surface glycoprotein CD1d1
UniProt Protein Name
Antigen-presenting glycoprotein CD1d1
Protein Family
UniProt Gene Name
Cd1d1
UniProt Synonym Gene Names
Cd1.1
UniProt Entry Name
CD1D1_MOUSE
Uniprot Description
CD1D: Antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.
Protein type: Lipid-binding; Apoptosis; Membrane protein, integral; Cell surface
Cellular Component: cell surface; membrane; lysosome; early endosome; late endosome; cytoplasm; plasma membrane; integral to membrane; endosome; external side of plasma membrane
Molecular Function: protein binding; histone binding; beta-2-microglobulin binding; exogenous lipid antigen binding; T cell receptor binding; lipid antigen binding; cell adhesion molecule binding; endogenous lipid antigen binding
Biological Process: antigen processing and presentation, endogenous lipid antigen via MHC class Ib; regulation of immune response; positive regulation of NK T cell activation; positive regulation of T cell mediated cytotoxicity; immune system process; positive regulation of interleukin-2 biosynthetic process; antigen processing and presentation; positive regulation of interleukin-4 biosynthetic process; antigen processing and presentation, exogenous lipid antigen via MHC class Ib; innate immune response; positive regulation of NK T cell differentiation; immune response; positive regulation of T cell proliferation; positive regulation of macrophage activation; positive thymic T cell selection; positive regulation of interferon-gamma biosynthetic process
Research Articles on CD1D
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Product Notes
The CD1D cd1d1 (Catalog #AAA8116449) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 532aa. AAA Biotech's CD1D can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the CD1D cd1d1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Gly30 5. It is sometimes possible for the material contained within the vial of "CD1D, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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