BACE2 cell lysate
Mouse BACE2 HEK293 Overexpression Lysate
Gene Names
Bace2; ARP1; BAE2; DRAP; AEPLC; ALP56; ASP21; CDA13; CEAP1; AI850424; 1110059C24Rik
Applications
Western Blot
Synonyms
BACE2; Mouse BACE2 HEK293 Overexpression Lysate; Mouse BACE2/Beta secretase 2 HEK293 Cell Lysate (WB positive control); beta-site APP-cleaving enzyme 2; Mouse 1110059C24Rik Overexpression Lysate; Mouse AEPLC Overexpression Lysate; Mouse AI850424 Overexpression Lysate; Mouse ALP56 Overexpression Lysate; Mouse ARP1 Overexpression Lysate; Mouse ASP21 Overexpression Lysate; Mouse BAE2 Overexpression Lysate; Mouse CDA13 Overexpression Lysate; Mouse CEAP1 Overexpression Lysate; Mouse DRAP Overexpression Lysate; BACE2 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
454aa
Sequence
Met1-Pro462
Applicable Applications for BACE2 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse BACE2 (Q9JL18) (Met1-Pro462) was expressed with a C-terminal polyhistidine tag.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for BACE2 cell lysate
BACE2, also known as beta secretase 2, belongs to the peptidase A1 family. It is a protease known to be an important enzyme involved in the cellular pathways. BACE2 has been shown to interact with GGA1 and GGA2. It is the major beta-secretase in vivo. BACE2 is located on chromosome 21 and may play a role in alzheimer's disease pathogenesis in down syndrome(DS). Overexpression of BACE2 by lentivirus markedly reduced amyloid beta protein production in primary neurons. Despite an extra copy of the BACE2 gene in DS and the increase of its transcription, BACE2 protein levels are unchanged.
This Mouse BACE2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of BACE2 protein from the overexpression lysate was verified.
This Mouse BACE2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of BACE2 protein from the overexpression lysate was verified.
References
Hussain I, et al. (2001) Prodomain processing of Asp1 (BACE2) is autocatalytic. J Biol Chem. 276(26):23322-8.Solans A, et al. (2000) A new aspartyl protease on 21q22.3, BACE2, is highly similar to Alzheimer's amyloid precursor protein beta-secretase. Cytogenet Cell Genet. 89(3-4): 177-84.Hussain I, et al. (2001) ASP1 (BACE2) cleaves the amyloid precursor protein at the beta-secretase site. Mol Cell Neurosci. 16(5):609-19.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55,800 Da
NCBI Official Full Name
beta-secretase 2
NCBI Official Synonym Full Names
beta-site APP-cleaving enzyme 2
NCBI Official Symbol
Bace2
NCBI Official Synonym Symbols
ARP1; BAE2; DRAP; AEPLC; ALP56; ASP21; CDA13; CEAP1; AI850424; 1110059C24Rik
NCBI Protein Information
beta-secretase 2; ASP1; asp 1; memapsin-1; theta-secretase; aspartyl protease 1; beta-site APP cleaving enzyme 2; membrane-associated aspartic protease 1; beta-site amyloid precursor protein cleaving enzyme 2
UniProt Protein Name
Beta-secretase 2
Protein Family
UniProt Gene Name
Bace2
UniProt Synonym Gene Names
ASP1; Asp 1; Beta-site APP cleaving enzyme 2
UniProt Entry Name
BACE2_MOUSE
Similar Products
Product Notes
The BACE2 bace2 (Catalog #AAA8117002) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 454aa. AAA Biotech's BACE2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the BACE2 bace2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Pro46 2. It is sometimes possible for the material contained within the vial of "BACE2, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
