Cadherin-16 Active Protein | CDH16 active protein

Cadherin-16 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120577

• Purity: >70% as determined by SDS-PAGE
• Synonyms: Cadherin-16; Cadherin-16 Protein; Human; Recombinant (His Tag); Human KSP-Cadherin/Cadherin-16/CDH16 Protein (His Tag); UNQ695/PRO1340 Protein; cadherin 16; CDH16 active protein

• Host: HEK293 Cells
• Purity/Purification: >70% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala786

• Species: Human
• Activity: Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells. When cells are added to CDH16-His coated plates (10ug/mL, 100uL/well), approximately 20-40% will adhere specifically after 60 minutes at 37 degree C.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Lys 19
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain of human CDH16 (NP_004053.1)(Met 1-Ala 786) was expressed, fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CDH16 active protein

Background: KSP-Cadherin/Cadherin-16 is a member of the cadherin superfamily, calcium-dependent, membrane-associated glycoproteins. The protein consists of an extracellular domain containing 6 cadherin domains, a transmembrane region and a truncated cytoplasmic domain but lacks the prosequence and tripeptide HAV adhesion recognition sequence typical of most classical cadherins. Expression is exclusively in kidney, where the protein functions as the principal mediator of homotypic cellular recognition, playing a role in the morphogenic direction of tissue development. KSP-Cadherin/Cadherin-16 can be detected at later stages of tubulogenesis during human renal development and in the distal tubules of adult kidneys, no expression was found by immunohistochemistry or Western blot analysis in RCC tumour tissues and several RCC cell lines. However, despite the lack of protein expression, mRNA synthesis of KSP-Cadherin/Cadherin-16 could be detected by reverse transcriptase-polymerase chain reaction analysis in all RCC tissues and most of the RCC cell lines studied, although at a reduced level. The loss of KSP-Cadherin/Cadherin-16 protein was only observed in the malignant part of the tumour kidneys, whereas in the normal part of the affected kidneys KSP-Cadherin/Cadherin-16 expression was clearly detected. These results indicate a downregulation of Ksp-cadherin in RCC and suggest a role for this cell adhesion molecule in tumour suppression.

Product Categories/Family for CDH16 active protein

Canonical Wnt Pathway

References

Thomson RB, et al. (1999) Immunolocalization of Ksp-cadherin in the adult and developing rabbit kidney. Am J Physiol. 277 (1): 146-56.Thedieck C, et al. (2005) Expression of Ksp-cadherin during kidney development and in renal cell carcinoma. Br J Cancer. 92(11): 2010-7.Bai Y, et al. (2002) Regulation of kidney-specific Ksp-cadherin gene promoter by hepatocyte nuclear factor-1beta. Am J Physiol Renal Physiol. 283(4): 839-51.

NCBI and Uniprot Product Information

• NCBI GI #: 4757956
• NCBI GeneID: 1014
• NCBI Accession #: NP_004053.1
• NCBI GenBank Nucleotide #: NM_004062.3
• UniProt Accession #: O75309; Q6UW93
• Molecular Weight: 89,923 Da
• NCBI Official Full Name: cadherin-16 isoform 1
• NCBI Official Synonym Full Names: cadherin 16, KSP-cadherin
• NCBI Official Symbol: CDH16
• NCBI Protein Information: cadherin-16; KSP-cadherin; OTTHUMP00000174814; OTTHUMP00000174815; kidney-specific cadherin
• UniProt Protein Name: Cadherin-16
• Protein Family: Cadherin
• UniProt Gene Name: CDH16
• UniProt Synonym Gene Names: Ksp-cadherin
• UniProt Entry Name: CAD16_HUMAN

Uniprot Description

CDH16: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: basolateral plasma membrane; integral to membrane

Molecular Function: calcium ion binding

Biological Process: calcium-dependent cell-cell adhesion; cell adhesion; homophilic cell adhesion