TNFRSF1A active protein

TNFRSF1A Protein, Rat, Recombinant (hFc Tag)

Cat. Num. AAA8123372

• Gene Names: Tnfrsf1a; Tnfr1; TNFR-1
• Purity: >90% as determined by SDS-PAGE
• Synonyms: TNFRSF1A; TNFRSF1A Protein; Rat; Recombinant (hFc Tag); Rat TNFR1/CD120a/TNFRSF1A Protein (Fc Tag); tumor necrosis factor receptor superfamily member 1A; TNFRSF1A active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala211

• Species: Rat
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ile 22
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the rat TNFRSF1A (P22934)(Met1-Ala211) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: 1. Measured by its ability to inhibit TNFalpha-mediated cytotoxicity in L-929 mouse fibroblast cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30ng/mL in the presence of 0.05ng/mL of ratTNFalpha.; 2. Immobilized mouse TNFa (80-235) at 10ug/ml (100uL/well) can bind rat TNFRSF1A-Fc. The EC50 of rat TNFRSF1A-Fc is 0.08-0.20ug/ml.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for TNFRSF1A active protein

Background: The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD120a (cluste of differentiation 120a), also known as TNFR1/TNFRSF1A, is a member of CD family, tumor necrosis factor receptor superfamily. CD120a is one of the most primary receptors for the tumor necrosis factor-alpha. It has been shown to be localized to both plasma membrane lipid rafts and the trans golgi complex with the help of the death domain (DD). CD120a can activate the transcription factor NF-kappaB, mediate apoptosis, and regulate inflammation processes.

Product Categories/Family for TNFRSF1A active protein

Neuroinflammation; Death Receptor Signaling

References

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.Cottin V, et al. (2002) Restricted localization of the TNF receptor CD120a to lipid rafts: a novel role for the death domain. The journal of immunology. 168: 4095-102.

NCBI and Uniprot Product Information

• NCBI GI #: 6981664
• NCBI GeneID: 25625
• NCBI Accession #: NP_037223.1
• NCBI GenBank Nucleotide #: NM_013091.1
• UniProt Accession #: P22934; Q5U1X6; Q91V30; Q91Y93
• Molecular Weight: 50,969 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 1A
• NCBI Official Synonym Full Names: TNF receptor superfamily member 1A
• NCBI Official Symbol: Tnfrsf1a
• NCBI Official Synonym Symbols: Tnfr1; TNFR-1
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 1A
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 1A
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: Tnfrsf1a
• UniProt Synonym Gene Names: Tnfr-1; Tnfr1; TNF-R1; TNF-RI; TNFR-I

NCBI Description

may play a role in TNFalpha mediated inflammatory response to spinal cord injury [RGD, Feb 2006]

Uniprot Description

Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis ().