Rabbit anti-Human ECGF1 Polyclonal Antibody | anti-ECGF1 antibody

ECGF1, ID (Thymidine Phosphorylase, TdRPase, TP, Platelet-derived Endothelial Cell Growth Factor, PD-ECGF, Gliostatin, TYMP) (FITC)

Cat. Num. AAA6473522

• Gene Names: TYMP; TP; ECGF; ECGF1; MNGIE; MEDPS1; MTDPS1; PDECGF; hPD-ECGF
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: ECGF1; Polyclonal Antibody; ID (Thymidine Phosphorylase; TdRPase; TP; Platelet-derived Endothelial Cell Growth Factor; PD-ECGF; Gliostatin; TYMP) (FITC); anti-ECGF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ECGF1.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ECGF1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide at the Center of human ECGF1 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-ECGF1(Center) Pab in HepG2 cell line lysates (35ug/lane). ECGF1(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ECGF1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-ECGF1 antibody

Antibodies; Enzymes; Phosphorylase

References

1. Stenman, G., Cytogenet. Cell Genet. 59(1), 22-23 (1992). 2. Jinfeng, M., Hepatogastroenterology 54(78), 1635-1640 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 4503445
• NCBI GeneID: 1890
• NCBI Accession #: NP_001944
• NCBI GenBank Nucleotide #: NM_001953.4
• UniProt Accession #: P19971
• Molecular Weight: 53kDa
• NCBI Official Full Name: thymidine phosphorylase isoform 1
• NCBI Official Synonym Full Names: thymidine phosphorylase
• NCBI Official Symbol: TYMP
• NCBI Official Synonym Symbols: TP; ECGF; ECGF1; MNGIE; MEDPS1; MTDPS1; PDECGF; hPD-ECGF
• NCBI Protein Information: thymidine phosphorylase
• UniProt Protein Name: Thymidine phosphorylase
• UniProt Gene Name: TYMP
• UniProt Synonym Gene Names: ECGF1; TP; PD-ECGF
• UniProt Entry Name: TYPH_HUMAN

NCBI Description

This gene encodes an angiogenic factor which promotes angiogenesis in vivo and stimulates the in vitro growth of a variety of endothelial cells. It has a highly restricted target cell specificity acting only on endothelial cells. Mutations in this gene have been associated with mitochondrial neurogastrointestinal encephalomyopathy. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Apr 2012]

Uniprot Description

ECGF1: May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro. Homodimer. Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family.

Protein type: Phosphorylase; EC 2.4.2.4; Xenobiotic Metabolism - drug metabolism - other enzymes; Transferase; Cytokine; Nucleotide Metabolism - pyrimidine; Motility/polarity/chemotaxis; DNA replication

Chromosomal Location of Human Ortholog: 22q13.33

Cellular Component: cytosol

Molecular Function: thymidine phosphorylase activity; growth factor activity; phosphorylase activity; transferase activity, transferring pentosyl groups; pyrimidine-nucleoside phosphorylase activity; platelet-derived growth factor receptor binding

Biological Process: mitochondrial genome maintenance; pyrimidine nucleoside catabolic process; pyrimidine base metabolic process; pyrimidine nucleotide metabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleoside salvage; angiogenesis; chemotaxis; DNA replication; cell differentiation

Disease: Mitochondrial Dna Depletion Syndrome 1 (mngie Type)