PD-1/PDCD1 Active Protein | PDCD1 active protein

Human CellExp PD-1 / PDCD1, mouse recombinant

Cat. Num. AAA845023

• Gene Names: Pdcd1; PD-1; Pdc1; Ly101
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: PD-1/PDCD1; Human CellExp PD-1 / PDCD1; mouse recombinant; PD; PD 1; PD 1 protein; PD 1 recombinant protein; mouse PD 1 protein; mouse recombinant PD 1 protein; mouse recombinant PD 1; mouse PD 1; recombinant PD 1; Human cell expressed PD 1; Human cell expressed PD 1 protein; Human cell expressed recombinant PD 1; Human cell expressed recombinant PD 1 protein; PD-1; PD-1 protein; PD-1 recombinant protein; mouse PD-1 protein; mouse recombinant PD-1 protein; mouse recombinant PD-1; mouse PD-1; recombinant PD-1; Human cell expressed PD-1; Human cell expressed PD-1 protein; Human cell expressed recombinant PD-1; Human cell expressed recombinant PD-1 protein; PD1; PD1 protein; PD1 recombinant protein; mouse PD1 protein; mouse recombinant PD1 protein; mouse recombinant PD1; mouse PD1; recombinant PD1; Human cell expressed PD1; Human cell expressed PD1 protein; Human cell expressed recombinant PD1; Human cell expressed recombinant PD1 protein; PDCD1; CD279; SLEB2; hPD-1; hPD-l; October-RP-14; PDCD; PDCD 1; PDCD 1 protein; PDCD 1 recombinant protein; mouse PDCD 1 protein; mouse recombinant PDCD 1 protein; mouse recombinant PDCD 1; mouse PDCD 1; recombinant PDCD 1; Human cell expressed PDCD 1; Human cell expressed PDCD 1 protein; Human cell expressed recombinant PDCD 1; Human cell expressed recombinant PDCD 1 protein; PDCD-1; PDCD-1 protein; PDCD-1 recombinant protein; mouse PDCD-1 protein; mouse recombinant PDCD-1 protein; mouse recombinant PDCD-1; mouse PDCD-1; recombinant PDCD-1; Human cell expressed PDCD-1; Human cell expressed PDCD-1 protein; Human cell expressed recombinant PDCD-1; Human cell expressed recombinant PDCD-1 protein; PDCD1 protein; PDCD1 recombinant protein; mouse PDCD1 protein; mouse recombinant PDCD1 protein; mouse recombinant PDCD1; mouse PDCD1; recombinant PDCD1; Human cell expressed PDCD1; Human cell expressed PDCD1 protein; Human cell expressed recombinant PDCD1; Human cell expressed recombinant PDCD1 protein; PDCD1 active protein

• Host: HEK293 cells
• Purity/Purification: >=95%
• Form/Format: Lyophilized from 0.22 mum filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.; Appearance: Lyophilized
• Sequence Length: 288

• Applicable Applications for PDCD1 active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Endotoxin Level: <1 EU/mug by LAL method
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized mouse PDCD1 Protein at 5 mug/ml can bind mouse PDL1 protein Fc Chimera with a linear range of 0.2-5 mug/ml when detected by HRP*-labeled Goat anti Human IgG, Fcgamma Fragment MAb.
• Results: Measured by its binding ability in a functional ELISA. Immobilized mouse PDCD1 Protein at 5 mug/ml can bind mouse PDL1 protein Fc Chimera with a linear range of 0.2-5 mug/ml when detected by HRP*-labeled Goat anti Human IgG, Fcgamma Fragment MAb.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for PDCD1 active protein

Background: Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-gamma. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-gamma by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-gamma secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer. This protein is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. It can also be used as an immunogen, as a protein standard, or in cell biology research applications.

Product Categories/Family for PDCD1 active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteins Proteins and Enzymes; Human cell expressed recombinant proteins Cytokines; Growth Factors & Hormones; CDsProteins and Enzymes; Cytokines; Growth Factors & Hormones; CDsCytokin

NCBI and Uniprot Product Information

• NCBI GI #: 6679239
• NCBI GeneID: 18566
• NCBI Accession #: NP_032824.1
• NCBI GenBank Nucleotide #: NM_008798.2
• UniProt Accession #: Q02242
• Molecular Weight: This protein is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 17.4 kDa. The predicted N-terminus is Ser 21. DTT-reduced Protein migrates as 25-45 kDa in SDS-PAGE due to glycosylation.
• NCBI Official Full Name: programmed cell death protein 1
• NCBI Official Synonym Full Names: programmed cell death 1
• NCBI Official Symbol: Pdcd1
• NCBI Official Synonym Symbols: PD-1; Pdc1; Ly101
• NCBI Protein Information: programmed cell death protein 1
• UniProt Protein Name: Programmed cell death protein 1
• Protein Family: Programmed cell death protein
• UniProt Gene Name: Pdcd1
• UniProt Synonym Gene Names: Pd1; Protein PD-1; mPD-1
• UniProt Entry Name: PDCD1_MOUSE

Uniprot Description

PDCD1: a single-pass type I membrane protein. A negative regulator of activated T cells, is upregulated in human and mouse 'exhausted' T cells during persistent viral infection. A marker of angioimmunoblastic T-cell lymphoma and germinal center associated T cells.

Protein type: Membrane protein, integral; Apoptosis

Cellular Component: membrane; integral to membrane; plasma membrane; external side of plasma membrane

Molecular Function: protein binding

Biological Process: immune system process; apoptosis; positive regulation of apoptosis; negative regulation of tolerance induction; negative regulation of apoptosis