HVEM/TNFRSF14 Active Protein | TNFRSF14 active protein

Human CellExp HVEM / TNFRSF14, human recombinant

Cat. Num. AAA844589

• Gene Names: TNFRSF14; TR2; ATAR; HVEA; HVEM; CD270; LIGHTR
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: HVEM/TNFRSF14; Human CellExp HVEM / TNFRSF14; human recombinant; TNFRSF14; ATAR; HVEA; HVEM; LIGHTR; TR2; Tumor necrosis factor receptor superfamily member 14; Herpesvirus entry mediator.; TNFRSF14 active protein

• Host: HEK293 cells
• Purity/Purification: >=95%
• Form/Format: Lyophilized from 0.22 mum filtered solution in 50 mM tris, 100 mM glycine, pH 7.0. Normally Mannitol or Trehalose is added as protectants before lyophilization.; Appearance: Lyophilized
• Sequence Length: 283

• Applicable Applications for TNFRSF14 active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Endotoxin Level: <1 EU/mug by LAL method
• Biological Activity: Measured by its ability to inhibit TNF-beta-mediated cytotoxicity using L929 Mouse fibrosarcoma cells. The ED50 for this effect is typically 2.5-10 mug/ml in the presence of 1 ng/ml of recombinant human TNF-beta.
• Results: Measured by its ability to inhibit TNF-beta-mediated cytotoxicity using L929 Mouse fibrosarcoma cells. The ED50 for this effect is typically 2.5-10 mug/ml in the presence of 1 ng/ml of recombinant human TNF-beta.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for TNFRSF14 active protein

Background: Herpesvirus entry mediator (HVEM), also known as TNFRSF14, TR2 (TNF receptor like molecule) and ATAR (another TRAF associated receptor), is a type I membrane protein belonging to the TNF/NGF receptor superfamily. HVEM expression has been detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. The extracellular domain of HVEM has been shown to interact directly with the herpes simplex virus envelope glycoprotein D (gD). Two TNF superfamily ligands, including the secreted TNFbeta (lymphotoxin alpha) and the membrane protein LIGHT (lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), have been shown to be the cellular ligands for HVEM. Besides HVEM, LIGHT can also interact with LTbetaR, the receptor for lymphotoxin alphabeta heterotrimer. The role of the HVEM LIGHT /LTbeta receptor ligand pair in immune function and herpesvirus pathobiology remains to be elucidated.

Product Categories/Family for TNFRSF14 active protein

Proteins and Enzymes; Human cell expressed recombinant proteinsCytokines; Growth Factors & Hormones; Human cell expressed recombinant proteinsProteins and Enzymes; Cytokines; Growth Factors & Hormones; TNFsProteins and Enzymes; Cytokines; Growth Factors &

NCBI and Uniprot Product Information

• NCBI GI #: 23200041
• NCBI GeneID: 8764
• NCBI Accession #: NP_003811.2
• NCBI GenBank Nucleotide #: NM_003820.3
• UniProt Accession #: Q92956; Q6IB95; Q8N634; Q8WXR1; Q96J31; Q9UM65; B3KW30; B9DI89
• Molecular Weight: This protein is a disulfide-linked homodimeric protein after removal of the signal peptide. The reduced monomer consists of 413 amino. rhHVEM-Fc, fused with the Fc region of human IgG1 at the C-terminus and has a calculated MW of 45.4 kDa expressed. Prote
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 14 isoform 1
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 14
• NCBI Official Symbol: TNFRSF14
• NCBI Official Synonym Symbols: TR2; ATAR; HVEA; HVEM; CD270; LIGHTR
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 14
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 14
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: TNFRSF14
• UniProt Synonym Gene Names: HVEA; HVEM; Herpesvirus entry mediator A; HveA; TR2
• UniProt Entry Name: TNR14_HUMAN

NCBI Description

This gene encodes a member of the TNF (tumor necrosis factor) receptor superfamily. The encoded protein functions in signal transduction pathways that activate inflammatory and inhibitory T-cell immune response. It binds herpes simplex virus (HSV) viral envelope glycoprotein D (gD), mediating its entry into cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

TNFRSF14: Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells.

Protein type: Cell cycle regulation; Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p36.32

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: viral receptor activity; protein binding; tumor necrosis factor receptor activity; ubiquitin protein ligase binding

Biological Process: defense response to Gram-positive bacterium; positive regulation of peptidyl-tyrosine phosphorylation; entry of virus into host cell; cell surface receptor linked signal transduction; tumor necrosis factor-mediated signaling pathway; T cell costimulation; positive regulation of cytokine secretion during immune response; negative regulation of alpha-beta T cell proliferation; immune response; defense response to Gram-negative bacterium