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Western Blot (WB) ((0.1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat Dnajc5 Polyclonal Antibody | anti-Dnajc5 antibody

Goat anti-Dnajc5 Antibody

Gene Names
Dnajc5; Csp; AU018536; 2610314I24Rik
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Dnajc5; Polyclonal Antibody; Goat anti-Dnajc5 Antibody; Goat Anti-Dnajc5 (aa177-191) Antibody; DnaJ (Hsp40) homolog; subfamily C; member 5; 2610314I24Rik; AU018536; Csp; cysteine string protein; dnaJ homolog subfamily C member 5; 2610314I24Rik antibody; AU018536 antibody; Csp antibody; cysteine string protein antibody; member 5 antibody; dnaJ homolog subfamily C member 5 antibody; Dnajc5 antibody; anti-Dnajc5 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
TQLTADSHPSYHTD
Sequence Length
198
Applicable Applications for anti-Dnajc5 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 21kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 22.1kDa according to NP_058055.1). Recommended concentration: 0.1-0.3ug/ml.
Immunogen
Peptide with sequence C-TQLTADSHPSYHTD, from the C Terminus of the protein sequence according to NP_058055.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((0.1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,101 Da
NCBI Official Full Name
dnaJ homolog subfamily C member 5
NCBI Official Synonym Full Names
DnaJ heat shock protein family (Hsp40) member C5
NCBI Official Symbol
Dnajc5
NCBI Official Synonym Symbols
Csp; AU018536; 2610314I24Rik
NCBI Protein Information
dnaJ homolog subfamily C member 5
UniProt Protein Name
DnaJ homolog subfamily C member 5
Protein Family
UniProt Gene Name
Dnajc5
UniProt Synonym Gene Names
CSP
UniProt Entry Name
DNJC5_MOUSE

Uniprot Description

DNAJC5: a cysteine string protein that inhibits N-type calcium channels which is blocked by huntingtin mutants. May have an important role in presynaptic function. May be involved in calcium-dependent neurotransmitter release at nerve endings. Modulates the maturation of cystic fibrosis transmembrane conductance regulator. Two splice-variant isoforms have been described.

Protein type: Vesicle

Cellular Component: lysosomal membrane; membrane; mitochondrion; plasma membrane; synaptic vesicle; terminal button

Molecular Function: ATP-dependent protein binding; glycoprotein binding; protein binding

Biological Process: negative regulation of neuron apoptosis

Research Articles on Dnajc5

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Product Notes

The Dnajc5 dnajc5 (Catalog #AAA422981) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Dnajc5 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's Dnajc5 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx 21kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 22.1kDa according to NP_058055.1). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the Dnajc5 dnajc5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: TQLTADSHPS YHTD. It is sometimes possible for the material contained within the vial of "Dnajc5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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