HDAC8 active protein

Human CellExp HDAC8, Human Recombinant, Active

Cat. Num. AAA849893

• Gene Names: HDAC8; HD8; WTS; RPD3; CDA07; CDLS5; MRXS6; HDACL1
• Purity: >=95% by SEC
• Synonyms: HDAC8; Human CellExp HDAC8; Human Recombinant; Active; HDACL1; HD8; Histone Deacetylase 8; CDA07; HDAC8 active protein

• Host: HEK293 cells
• Purity/Purification: >=95% by SEC
• Form/Format: Liquid. In 30mM HEPES, 140mM NaCl, 10mM KCl, 3% glycerol, 0.25mM TCEP, pH7.5

• Gene Source: Human
• Biological Activity: Deacetylation activity was determined using the Boc-K(TFA)-AMC substrate. Reactions were carried outin a 384-well plate in 20uL ofreaction buffer comprisingof50 mM HEPES, 140 mM NaCl, 10 mM KCl, 1 mg/ml bovine serum albumin (BSA), and 1 mM TCEP, pH 7.4, for 30 min at 37 degree C. Thereaction was stopped by addition of 20ul of trypsin solution (2mg/mltrypsin) and thefluorescence signal of released AMC was quantified using a fluorometer. The enzymekinetic values were calculated by non-linear regression analysis.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Store at -80 degree C; Thaw on ice, and aliquot into smaller working quantities to avoid multiple freeze/thaw cycles.; Shipping: Dry Ice; Shelf life: ~12 months

Testing Data

Related Product Information for HDAC8 active protein

Human Histone Deacetylase 8 (HDAC8) is a member of the class I Histone Deacetylases. HDACs are important enzymes for the transcriptional regulation of gene expression in eukaryotic cells. HDACs catalyze the removal of acetyl groups from lysines near the N-termini of histones. Human HDACs have been implicated in a variety of human diseases such as cardiomyopathy, osteodystrophy, neurodegenerative disorders, aging and cancer. Expression of HDAC8 is restricted to cells showing smooth muscle differentiation in normal human tissue and is a novel marker of smooth muscle differentiation. Like other class I and II HDAC members, the activity of HDAC8 is sensitive to HDAC inhibitor Trichostatin A (TSA) .

Product Categories/Family for HDAC8 active protein

Cancer Research; Epigenetic Regulation; Eraser Enzymes; Histone Deacetylases (HDACs); HDAC Enzymes & Substrates

NCBI and Uniprot Product Information

• NCBI GI #: 29839394
• NCBI GeneID: 55869
• UniProt Accession #: Q9BY41; Q86VC8; Q9NP76; Q9NYH4; A6ND12; A6ND61; A6NET3; A6NJR3; A8MQ62; B4DKN0; B4DV22
• Molecular Weight: 377
• NCBI Official Full Name: Histone deacetylase 8
• NCBI Official Synonym Full Names: histone deacetylase 8
• NCBI Official Symbol: HDAC8
• NCBI Official Synonym Symbols: HD8; WTS; RPD3; CDA07; CDLS5; MRXS6; HDACL1
• NCBI Protein Information: histone deacetylase 8; histone deacetylase-like 1
• UniProt Protein Name: Histone deacetylase 8
• UniProt Gene Name: HDAC8
• UniProt Synonym Gene Names: HDACL1; HD8
• UniProt Entry Name: HDAC8_HUMAN

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

HDAC8: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility. Belongs to the histone deacetylase family. HD type 1 subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.5.1.98; Hydrolase

Chromosomal Location of Human Ortholog: Xq13

Cellular Component: nucleoplasm; nuclear chromosome; histone deacetylase complex; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function: NAD-dependent histone deacetylase activity (H3-K9 specific); NAD-dependent histone deacetylase activity (H3-K14 specific); metal ion binding; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription factor binding

Biological Process: chromatin assembly or disassembly; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; sister chromatid cohesion; mitotic cell cycle; chromatin modification; negative regulation of transcription from RNA polymerase II promoter

Disease: Wilson-turner X-linked Mental Retardation Syndrome