HDAC6 active protein

Human CellExp HDAC6, Human Recombinant, Active

Cat. Num. AAA849892

• Gene Names: HDAC6; HD6; JM21
• Purity: >=90% by SEC
• Synonyms: HDAC6; Human CellExp HDAC6; Human Recombinant; Active; KIAA0901; JM21; HD6; HDAC6 active protein

• Host: HEK293 cells
• Purity/Purification: >=90% by SEC
• Form/Format: Liquid. In 30mM HEPES, 140mM NaCl, 10mM KCl, 3% glycerol, 0.25mM TCEP, pH7.4

• Gene Source: Human
• Biological Activity: Deacetylation activity was determined using GAK (Ac) -AMC, Boc-K (Ac) -AMC and Fluor-de-Lys (RHK-K (Ac) -AMC) substrates. Reactions were carried outin a 384-well plate in 20uL ofreaction buffer comprisingof50 mM HEPES, 140 mM NaCl, 10 mM KCl, 1 mg/ml bovine serum albumin (BSA), and 1 mM TCEP, pH 7.4, for 30 min at 37 degree C. Thereaction was stopped by addition of 20 ul of trypsin solution (2mg/mltrypsin) and a fluorescence signal of released AMC was quantified using a fluorometer. The enzymekinetic values were calculated by non-linear regression analysis.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Store at -80 degree C; Thaw on ice, and aliquot into smaller working quantities to avoid multiple freeze/thaw cycles.; Shipping: Dry Ice; Shelf life: ~12 months

Testing Data

Related Product Information for HDAC6 active protein

HDAC6 is a Class II HDAC. It is unique among human HDACs in having two full deacetylase domains. As a tubulin deacetylase, HDAC6 may act to promote autophagic clearing of Huntington aggregates and retard HIV1 infection. It can shuttle between the nucleus and cytoplasm, suggesting potential extra nuclear functions by regulating the acetylation status of non-histone substrates. By modifying chromatin structure and other non-histone proteins, HDACs play important roles in controlling complex biological events, including cell development, differentiation, programmed cell death, angiogenesis, and inflammation. Considering these major roles, it is conceivable that dysregulation of HDACs and subsequent imbalance of acetylation and deacetylation may be involved in the pathogenesis of various diseases, including cancer and inflammatory diseases. Regulates the acetylation status of non-histone substrates

Product Categories/Family for HDAC6 active protein

Cancer Research; Epigenetic Regulation; Eraser Enzymes; Histone Deacetylases (HDACs); HDAC Enzymes & Substrates

NCBI and Uniprot Product Information

• NCBI GI #: 13128864
• NCBI GeneID: 10013
• NCBI Accession #: NP_006035.2
• NCBI GenBank Nucleotide #: NM_006044.2
• UniProt Accession #: Q9UBN7; O94975; Q6NT75; Q7L3E5; Q96CY0
• Molecular Weight: 131,419 Da
• NCBI Official Full Name: histone deacetylase 6
• NCBI Official Synonym Full Names: histone deacetylase 6
• NCBI Official Symbol: HDAC6
• NCBI Official Synonym Symbols: HD6; JM21
• NCBI Protein Information: histone deacetylase 6
• UniProt Protein Name: Histone deacetylase 6
• UniProt Gene Name: HDAC6
• UniProt Synonym Gene Names: KIAA0901; HD6
• UniProt Entry Name: HDAC6_HUMAN

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription. [provided by RefSeq, Jul 2008]

Uniprot Description

HDAC6: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. Interacts with CBFA2T3, HDAC11 and SIRT2. Interacts with F-actin. Interacts with BBIP10. Under proteasome impairment conditions, interacts with UBD via its histone deacetylase 1 and UBP-type zinc-finger regions. Interacts with CYLD. Interacts with ZMYND15. Belongs to the histone deacetylase family. HD type 2 subfamily.

Protein type: Nuclear receptor co-regulator; EC 3.5.1.98; Ubiquitin conjugating system; Deacetylase

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: microtubule; dendrite; histone deacetylase complex; leading edge; perikaryon; caveola; inclusion body; cytosol; nucleoplasm; dynein complex; microtubule associated complex; cytoplasmic microtubule; axon; perinuclear region of cytoplasm; cytoplasm; nucleus

Molecular Function: zinc ion binding; histone deacetylase binding; microtubule binding; beta-tubulin binding; beta-catenin binding; misfolded protein binding; Hsp90 protein binding; actin binding; protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); enzyme binding; NAD-dependent histone deacetylase activity (H3-K14 specific); tubulin deacetylase activity; ubiquitin protein ligase binding; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; polyubiquitin binding; tau protein binding; alpha-tubulin binding

Biological Process: negative regulation of proteolysis; response to misfolded protein; protein polyubiquitination; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; positive regulation of signal transduction; transcription, DNA-dependent; regulation of fat cell differentiation; negative regulation of microtubule depolymerization; macroautophagy; organelle organization and biogenesis; response to toxin; misfolded or incompletely synthesized protein catabolic process; histone deacetylation; regulation of gene expression, epigenetic; response to organic substance; intracellular protein transport; protein complex disassembly; protein amino acid deacetylation; lysosome localization; negative regulation of oxidoreductase activity; regulation of receptor activity; negative regulation of transcription, DNA-dependent; negative regulation of protein complex disassembly

Disease: Chondrodysplasia With Platyspondyly, Distinctive Brachydactyly, Hydrocephaly, And Microphthalmia