FGFRL1 active protein

FGFRL1 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122591

• Gene Names: Fgfrl1; FGFR5; FGFR5beta; FGFR5gamma
• Purity: >90% as determined by SDS-PAGE
• Synonyms: FGFRL1; FGFRL1 Protein; Mouse; Recombinant (His Tag); Mouse FGFRL1/FGFR5 Protein (His Tag); FGFR5 Protein; FGFR5beta Protein; FGFR5gamma Protein; fibroblast growth factor receptor-like 1; FGFRL1 active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Pro374

• Species: Mouse
• Activity: Measured by its binding ability in a functional ELISA.; 1. Immobilized mouse at 10ug/ml (100uL/well) can bind mouse FGFR5. The EC50 of mouse FGFR5 is 0.34ug/ml.; 2. Immobilized human FGF1 at 10ug/ml (100uL/well) can bind mouse FGFR5 with a linear range of 0.08-2ug/ml.; 3. Immobilized human bFGF at 5ug/ml (100uL/well) can bind mouse FGFR5. The EC50 of mouse FGFR5 is 0.22ug/ml.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ala 21
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse FGFRL1 (NP_473412.1)(Met 1-Pro 374) precursor was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for FGFRL1 active protein

Background: Fibroblast growth factor receptor-like 1 (FGFRL1) also known as Fibroblast growth factor receptor 5 (FGFR5), is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. A unique feature of FGFRL1/FGFR5 is that it does not contain an intracellular tyrosine kinase domain. Some muscle types, including the muscles of the tongue and the diaphragm, express FGFRL1/FGFR5 at relatively high level. In contrast, the heart and the skeletal muscles of the limbs, as well as many other organs (brain, lung, liver, kidney, gut) express Fgfrl1 only at basal level. It is conceivable that FGFRL1/FGFR5 interacts with other Fgfrs, which are expressed in cartilage and muscle, to modulate FGF signaling.

Product Categories/Family for FGFRL1 active protein

Receptor Tyrosine Kinases (RTKs)

References

Wiedemann M, et al. (2000) Characterization of a novel protein (FGFRL1) from human cartilage related to FGF receptors. Genomics. 69(2): 275-9.
Trueb B, et al. (2006) Expression of FGFRL1, a novel fibroblast growth factor receptor, during embryonic development. Int J Mol Med. 17(4): 617-20.
Wiedemann M, et al. (2001) The mouse Fgfrl1 gene coding for a novel FGF receptor-like protein. Biochim Biophys Acta. 1520(3): 247-50.
Trueb B, et al. (2003) Characterization of FGFRL1, a novel fibroblast growth factor (FGF) receptor preferentially expressed in skeletal tissues. J Biol Chem. 278(36): 33857-65.

NCBI and Uniprot Product Information

• NCBI GI #: 16905101
• NCBI GeneID: 116701
• NCBI Accession #: NP_473412.1
• NCBI GenBank Nucleotide #: NM_054071.2
• UniProt Accession #: Q91V87; Q920C2; Q920C3
• Molecular Weight: 46,981 Da
• NCBI Official Full Name: fibroblast growth factor receptor-like 1 isoform 1
• NCBI Official Synonym Full Names: fibroblast growth factor receptor-like 1
• NCBI Official Symbol: Fgfrl1
• NCBI Official Synonym Symbols: FGFR5; FGFR5beta; FGFR5gamma
• NCBI Protein Information: fibroblast growth factor receptor-like 1
• UniProt Protein Name: Fibroblast growth factor receptor-like 1
• Protein Family: Fibroblast growth factor receptor
• UniProt Gene Name: Fgfrl1
• UniProt Synonym Gene Names: Fgfr5; FGF receptor-like protein 1; FGFR-5
• UniProt Entry Name: FGRL1_MOUSE

Uniprot Description

Function: Has a negative effect on cell proliferation

By similarity. Ref.4

Subunit structure: Interacts with FGF2 with a low affinity. Ref.1

Subcellular location: Cell membrane; Single-pass membrane protein

Potential.

Tissue specificity: Highly expressed in the kidney, brain and lung. Weakly expressed in the muscle, thymus, lymph node, stomach, intestine, colon and liver. Expressed in fetal cartilaginous structures like the nasal cartilage, the ribs and the sternum as well as in the cartilaginous rudiments of developing bones such as the vertebrae and the pelvic bone. High expression is found in the muscles of the tongue and the diaphragm. Ref.1 Ref.4

Developmental stage: First detected at embryonic day 7 and became clearly visible at day 11 and increased until day 17. Ref.2

Sequence similarities: Contains 3 Ig-like C2-type (immunoglobulin-like) domains.