Fas/CD95 Active Protein | FAS active protein

Fas/CD95 Protein, Rat, Recombinant (hFc Tag)

Cat. Num. AAA8123339

• Gene Names: Fas; Tnfrsf6
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Fas/CD95; Fas/CD95 Protein; Rat; Recombinant (hFc Tag); Rat FAS/CD95/APO-1/TNFRSF6 Protein (Fc Tag); Fas cell surface death receptor; FAS active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys170

• Species: Rat
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 22
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the rat FAS (NP_631933.2)(Met1-Lys170) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its binding ability in a functional ELISA. Immobilized human His-FASLG at 10ug/ml (100uL/well) can bind rat FAS-Fc. The EC50 of rat FAS-Fc is 0.22-0.52ug/ml.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for FAS active protein

Background: CD95 (APO-1/Fas) is an important inducer of the extrinsic apoptosis signaling pathway and therapy induced apoptosis of many tumor cells has been linked to the activity of CD95. is a prototype death receptor characterized by the presence of an 80 amino acid death domain in its cytoplasmic tail. This domain is essential for the recruitment of a number of signaling components upon activation by either agonistic anti-CD95 antibodies or cognate CD95 ligand that initiate apoptosis. The complex of proteins that forms upon triggering of CD95 is called the death-inducting signaling complex (DISC). The DISC consists of an adaptor protein and initiator caspases and is essential for induction of apoptosis. CD95 is also crucial for the negative selection of B cells within the germinal center (GC). Impairment of CD95-mediated apoptosis results in defective affinity maturation and the persistence of autoreactive B-cell clones. Changes in the expression of CD95 and/or its ligand CD95L are frequently found in human cancer. The downregulation or mutation of CD95 has been proposed as a mechanism by which cancer cells avoid destruction by the immune system through reduced apoptosis sensitivity. Thus, CD95 has therefore been viewed as a tumor suppressor. CD95 has been reported to be involved in the activation of NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and the alternate pathways for CTL-mediated cytotoxicity. Accordingly, this protein is implicated in the pathogenesis of various malignancies and diseases of the immune system. The CD95/CD95L system was implicated in the etiology of inflammatory bowel disease (IBD) based, primarily, on the finding that CD95 is highly expressed in the intestinal epithelial cells and that epithelial apoptosis is increased in IBD.

Product Categories/Family for FAS active protein

Tumor Suppressors; Death Receptor Signaling

References

Mschen M, et al. (2002) The origin of CD95-gene mutations in B-cell lymphoma. Trends Immunol. 23(2): 75-80.Peter ME, et al. (2003) The CD95(APO-1/Fas) DISC and beyond. Cell Death Differ. 10(1): 26-35.Peter ME, et al. (2005) Does CD95 have tumor promoting activities Biochim Biophys Acta. 1755(1): 25-36.Chen L, et al. (2010) Cell death in the colonic epithelium during inflammatory bowel diseases: CD95/Fas and beyond. Inflamm Bowel Dis. 16(6): 1071-6.

NCBI and Uniprot Product Information

• NCBI GI #: 59624977
• NCBI GeneID: 246097
• NCBI Accession #: NP_631933.2
• NCBI GenBank Nucleotide #: NM_139194.2
• UniProt Accession #: Q63199
• Molecular Weight: 36,835 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 6
• NCBI Official Synonym Full Names: Fas cell surface death receptor
• NCBI Official Symbol: Fas
• NCBI Official Synonym Symbols: Tnfrsf6
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 6; CD95 antigen; Fas receptor; apo-1 antigen; FASLG receptor; Fas antigen (ATP1); apoptosis-mediating surface antigen FAS; Fas (TNF receptor superfamily, member 6); Tumor necrosis factor receptor superfami
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 6
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: Fas
• UniProt Synonym Gene Names: Apt1; Tnfrsf6
• UniProt Entry Name: TNR6_RAT

NCBI Description

Tnfsf6/Fasl receptor [RGD, Feb 2006]

Uniprot Description

Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both ().