EphA3 active protein

EphA3 Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8123141

• Gene Names: Epha3; Hek; Cek4; ETK1; End3; Hek4; Mek4; Tyro4; AW492086
• Purity: >90% as determined by SDS-PAGE
• Synonyms: EphA3; EphA3 Protein; Mouse; Recombinant (hFc Tag); Mouse EphA3 Protein (Fc Tag); AW492086 Protein; Cek4 Protein; End3 Protein; ETK1 Protein; Hek Protein; Hek4 Protein; Mek4 Protein; Tyro4 Protein; EPH receptor A3; EphA3 active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-His541

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 21
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse EPHA3 (NP_034270.1)(Met1-His541) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its binding ability in a functional ELISA. Immobilized mouse EFNA5-His at 10ug/ml (100uL/well) can bind mouse EPHA3-Fc. The EC50 of rat mouse EPHA3-Fc is 12.3-28.9ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized mouse EFNA5-His at 10 ug/ml (100 ul/well) can bind mouse EPHA3-Fc, The EC50 of rat mouse EPHA3-Fc is 12.3-28.9 ng/ml.)

Related Product Information for EphA3 active protein

Background: EPHA3 gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EPHA3 gene encodes a protein that binds ephrin-A ligands. EPHA3 is involved in the retinotectal mapping of neurons. It may also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development.

Product Categories/Family for EphA3 active protein

Actin Dynamics Signaling Pathway

References

Holder N, et al. (1999) Eph receptors and ephrins: effectors of morphogenesis. Development. 126(10):2033-44.
Wilkinson DG. (2000) Eph receptors and ephrins: regulators of guidance and assembly. Int Rev Cytol. 196:177-244.
Xu Q, et al. (2001) Roles of Eph receptors and ephrins in segmental patterning. Philos Trans R Soc Lond B Biol Sci. 355(1399):993-1002.

NCBI and Uniprot Product Information

• NCBI GI #: 31982448
• NCBI GeneID: 13837
• NCBI Accession #: NP_034270.1
• NCBI GenBank Nucleotide #: NM_010140.3
• UniProt Accession #: P29319
• Molecular Weight: 109,955 Da
• NCBI Official Full Name: ephrin type-A receptor 3
• NCBI Official Synonym Full Names: Eph receptor A3
• NCBI Official Symbol: Epha3
• NCBI Official Synonym Symbols: Hek; Cek4; ETK1; End3; Hek4; Mek4; Tyro4; AW492086
• NCBI Protein Information: ephrin type-A receptor 3; EK4; EPH-like kinase 4; tyrosine-protein kinase TYRO4; tyrosine-protein kinase receptor ETK1
• UniProt Protein Name: Ephrin type-A receptor 3
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: Epha3
• UniProt Synonym Gene Names: Etk1; Mek4; Tyro4; EK4; mEK4
• UniProt Entry Name: EPHA3_MOUSE

Uniprot Description

EphA3: a receptor tyrosine kinase of the Eph family. Receptor for members of the ephrin-A family: binds to ephrin-A2, -A3, -A4 and -A5The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. Two point mutations seen in a survey of colorectal tumors. Soluble receptors reduce tumor growth and angiogenesis in mouse models. Two alternatively spliced isoforms of EphA3 have been described.

Protein type: Kinase, protein; Protein kinase, TK; Membrane protein, integral; Protein kinase, tyrosine (receptor); EC 2.7.10.1; TK group; Eph family

Cellular Component: membrane; integral to plasma membrane; early endosome; extracellular region; plasma membrane; integral to membrane

Molecular Function: transferase activity; GPI-linked ephrin receptor activity; protein-tyrosine kinase activity; ephrin receptor activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding; protein kinase activity

Biological Process: cell migration; peptidyl-tyrosine phosphorylation; regulation of actin cytoskeleton organization and biogenesis; regulation of focal adhesion formation; ephrin receptor signaling pathway; motor axon guidance; protein amino acid phosphorylation; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway