CD85J/LILRB1 Active Protein | LILRB1 active protein

Human CellExp CD85J / LILRB1, human recombinant

Cat. Num. AAA845081

• Gene Names: LILRB1; ILT2; LIR1; MIR7; CD85J; ILT-2; LIR-1; MIR-7
• Applications: SDS-Page
• Purity: >= 95% by SDS-PAGE gel
• Synonyms: CD85J/LILRB1; Human CellExp CD85J / LILRB1; human recombinant; CD85J; LILRB1; CD85; ILT2; LIR1; MIR7; LILRB1 active protein

• Host: HEK293 cells
• Purity/Purification: >= 95% by SDS-PAGE gel
• Form/Format: Lyophilized from 0.22 mum filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
• Sequence Length: 652

• Applicable Applications for LILRB1 active protein: SDS-PAGE
• Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: Store at -20°C. After reconstitution, aliquot and store at -20°C or -70°C for up to 3 months. Avoid repeated freezing and thawing cycles.

Testing Data

(Human recombinant CD85J/LILRB1 His-tag)

Related Product Information for LILRB1 active protein

Background: CD85 antigen-like family member J (CD85J) also known as Leukocyte immunoglobulin-like receptor subfamily B member 1 (LILRB1 or LIR-1), Immunoglobulin-like transcript 2 (ILT-2), Monocyte/macrophage immunoglobulin-like receptor 7 (MIR7), which belongs to leukocyte immunoglobulin-like receptor (LIR) family. CD85J / LILRB1 Contains 4 Ig-like C2-type (immunoglobulin-like) domains. CD85J / LIR-1 is expressed predominantly on B-cells and monocytes. CD85J is receptor for class I MHC antigens and recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles and is also receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. CD85J / LILRB1 interaction with HLA-B or HLA-E leads to inhibition of the signal triggered by FCER1A and inhibits serotonin release. CD85J / LILRB1 inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.

Product Categories/Family for LILRB1 active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteins Proteins and Enzymes; Human cell expressed recombinant proteins Proteins and Enzymes; Cytokines; Growth Factors & Hormones; CDsCytokines; Growth Factors & Hormones; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 612149791
• NCBI GeneID: 10859
• NCBI Accession #: NP_001075106.2
• NCBI GenBank Nucleotide #: NM_001081637.2
• UniProt Accession #: Q8NHL6; O75024; O75025; Q8NHJ9; Q8NHK0; A2IXV4; A8MXT0
• Molecular Weight: This protein is fused to the 6x His tag at the C-terminus and has a calculated MW of 49 kDa. The protein migrates as 55-70 kDa under reducing conditions (SDS-PAGE) due to glycosylation.
• NCBI Official Full Name: leukocyte immunoglobulin-like receptor subfamily B member 1 isoform 2
• NCBI Official Synonym Full Names: leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1
• NCBI Official Symbol: LILRB1
• NCBI Official Synonym Symbols: ILT2; LIR1; MIR7; CD85J; ILT-2; LIR-1; MIR-7
• NCBI Protein Information: leukocyte immunoglobulin-like receptor subfamily B member 1
• UniProt Protein Name: Leukocyte immunoglobulin-like receptor subfamily B member 1
• Protein Family: Leukocyte immunoglobulin-like receptor subfamily
• UniProt Gene Name: LILRB1
• UniProt Synonym Gene Names: ILT2; LIR1; MIR7; LIR-1; Leukocyte immunoglobulin-like receptor 1; ILT-2; MIR-7
• UniProt Entry Name: LIRB1_HUMAN

NCBI Description

This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

LILRB1: Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. Engagement of LILRB1 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. Interaction with HLA-B or HLA-E leads to inhibition of the signal triggered by FCER1A and inhibits serotonin release. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: cytoplasm; extracellular region; integral to membrane; plasma membrane; external side of plasma membrane

Molecular Function: protein homodimerization activity; MHC class I receptor activity; MHC class I protein binding; HLA-A specific inhibitory MHC class I receptor activity; SH2 domain binding; HLA-B specific inhibitory MHC class I receptor activity; protein phosphatase 1 binding

Biological Process: immune response-inhibiting cell surface receptor signaling pathway; positive regulation of apoptosis; signal transduction; negative regulation of interferon-gamma biosynthetic process; negative regulation of cell cycle; negative regulation of serotonin secretion; negative regulation of alpha-beta T cell activation; negative regulation of T cell proliferation; T cell proliferation during immune response; receptor internalization; negative regulation of interferon-gamma production; negative regulation of T cell mediated cytotoxicity; interferon-gamma production; negative regulation of tumor necrosis factor biosynthetic process; positive regulation of cytolysis; Fc receptor mediated inhibitory signaling pathway; defense response to virus; regulation of immune response; negative regulation of calcium ion transport; negative regulation of mononuclear cell proliferation; negative regulation of endocytosis; response to virus; negative regulation of natural killer cell mediated cytotoxicity; negative regulation of cytokine secretion during immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of defense response to virus by host