LILRB1/CD85j/ILT2 recombinant protein

Recombinant human LILRB1/CD85j/ILT2 protein

Cat. Num. AAA201278

• Gene Names: LILRB1; ILT2; LIR1; MIR7; CD85J; ILT-2; LIR-1; MIR-7
• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: LILRB1/CD85j/ILT2; Recombinant human LILRB1/CD85j/ILT2 protein; Leukocyte immunoglobulin-like receptor subfamily B member 1; LIR-1; LIR1; Leukocyte immunoglobulin-like receptor 1; CD85 antigen-like family member J; Immunoglobulin-like transcript 2; ILT-2; ILT2; Ig-like transcript 2; Monocyte/macrophage immunoglobulin-like receptor 7; MIR-7; MIR7; CD_antigen; CD85j; PIRB; PIR-B; leucocyte Ig-like receptor B1; leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform; leukocyte immunoglobulin-like receptor; subfamily B (with TM and ITIM domains); member 1; monocyte/macrophage immunoglobulin-like receptor 7; myeloid inhibitory receptor 7; LILRB1/CD85j/ILT2 recombinant protein

• Host: Baculovirus
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 0.25mg/ml (determined by absorbance at 280nm) (varies by lot)
• Sequence Positions: 24-461aa
• Sequence: GHLPKPTLWA EPGSVITQGS PVTLRCQGGQ ETQEYRLYRE KKTAPWITRI PQELVKKGQF PIPSITWEHT GRYRCYYGSD TAGRSESSDP LELVVTGAYI KPTLSAQPSP VVNSGGNVTL QCDSQVAFDG FILCKEGEDE HPQCLNSQPH ARGSSRAIFS VGPVSPSRRW WYRCYAYDSN SPYEWSLPSD LLELLVLGVS KKPSLSVQPG PIVAPEETLT LQCGSDAGYN RFVLYKDGER DFLQLAGAQP QAGLSQANFT LGPVSRSYGG QYRCYGAHNL SSEWSAPSDP LDILIAGQFY DRVSLSVQPG PTVASGENVT LLCQSQGWMQ TFLLTKEGAA DDPWRLRSTY QSQKYQAEFP MGPVTSAHAG TYRCYGSQSS KPYLLTHPSD PLELVVSGPS GGPSSPTTGP TSTSGPEDQP LTPTGSDPQS GLGRHLGV

• Applicable Applications for LILRB1/CD85j/ILT2 recombinant protein: SDS-PAGE, Bioactivity
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Human
• Tag: His-Tag
• Predicted N-term: Gly24
• Bioactivity: Measured by the ability of the immobilized protein to support the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to LILRB1 coated plates 5ug/ml. This effect is more to 50%.
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for LILRB1/CD85j/ILT2 recombinant protein

LILRB1, also known as leukocyte immunoglobulin like receptor B1, is a member of the leukocyte immunoglobulinlike receptor (LIR) family. The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. It is also expressed on the surface of B cells and monocytes, as well as subsets of NK cells, memory/effector CD8+ T cells, gamma delta T cells, and monocyte-derived dendritic cells. It binds to MHC-I as well as non-classical MHC-I molecules and the MHC-I mimetic UL18 encoded by cytomegalovirus. Recombinant human LILRB1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for LILRB1/CD85j/ILT2 recombinant protein

Immunology; Proteins

References

Colonna, M. et al. (1997) J. Exp. Med. 186:1809-1818. Cosman, D. et al. (1997) Immunity 7:273-282. Ince, M.N. et al. (2004) Immunology 112:531-542. M Colonna et al. (1998) Immunology 7:3096-3100.

NCBI and Uniprot Product Information

• NCBI GI #: 612149789
• NCBI GeneID: 10859
• NCBI Accession #: NP_006660.4
• NCBI GenBank Nucleotide #: NM_006669.6
• UniProt Accession #: Q8NHL6; O75024; O75025; Q8NHJ9; Q8NHK0; A2IXV4; A8MXT0
• Molecular Weight: 49,356 Da
• NCBI Official Full Name: leukocyte immunoglobulin-like receptor subfamily B member 1 isoform 1
• NCBI Official Synonym Full Names: leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1
• NCBI Official Symbol: LILRB1
• NCBI Official Synonym Symbols: ILT2; LIR1; MIR7; CD85J; ILT-2; LIR-1; MIR-7
• NCBI Protein Information: leukocyte immunoglobulin-like receptor subfamily B member 1; Ig-like transcript 2; CD85 antigen-like family member J; monocyte/macrophage immunoglobulin-like receptor 7; leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform
• UniProt Protein Name: Leukocyte immunoglobulin-like receptor subfamily B member 1
• UniProt Gene Name: LILRB1
• UniProt Synonym Gene Names: ILT2; LIR1; MIR7; LIR-1; Leukocyte immunoglobulin-like receptor 1; ILT-2; MIR-7
• UniProt Entry Name: LIRB1_HUMAN

Uniprot Description

LILRB1: Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. Engagement of LILRB1 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. Interaction with HLA-B or HLA-E leads to inhibition of the signal triggered by FCER1A and inhibits serotonin release. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: cytoplasm; extracellular region; plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein homodimerization activity; MHC class I receptor activity; MHC class I protein binding; HLA-A specific inhibitory MHC class I receptor activity; SH2 domain binding; HLA-B specific inhibitory MHC class I receptor activity; protein phosphatase 1 binding

Biological Process: immune response-inhibiting cell surface receptor signaling pathway; regulation of immune response; negative regulation of calcium ion transport; negative regulation of mononuclear cell proliferation; positive regulation of apoptosis; negative regulation of endocytosis; response to virus; negative regulation of natural killer cell mediated cytotoxicity; signal transduction; negative regulation of cytokine secretion during immune response; negative regulation of interferon-gamma biosynthetic process; negative regulation of cell cycle; negative regulation of serotonin secretion; negative regulation of alpha-beta T cell activation; negative regulation of T cell proliferation; T cell proliferation during immune response; receptor internalization; negative regulation of interferon-gamma production; negative regulation of T cell mediated cytotoxicity; interferon-gamma production; negative regulation of tumor necrosis factor biosynthetic process; Fc receptor mediated inhibitory signaling pathway; positive regulation of cytolysis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of defense response to virus by host; defense response to virus