SDS-Page
CD155/PVR Active Protein | PVR active protein
CD155/PVR Protein, Rhesus, Recombinant (His Tag)
Purity
>98% as determined by SDS-PAGE
Synonyms
CD155/PVR; CD155/PVR Protein; Rhesus; Recombinant (His Tag); Rhesus CD155/PVR Protein (His Tag); poliovirus receptor; PVR active protein
Host
HEK293 Cells
Purity/Purification
>98% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Asn343
Species
Rhesus
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Asp 28
Tag
C-His
Protein Construction
A DNA sequence encoding the rhesus PVR (NP_001036851.1) extracellular domain (Met 1-Asn 343) was expressed with a C-terminal polyhistidine tag.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Activity
1. Measured by its binding ability in a functional ELISA. Immobilized Rhesus CD155 at 2ug/ml (100uL/well) can bind human CD226 with a linear range of 1.28-32ng/mL.
2. Immobilized TIGIT Protein, Human, Recombinant (mFc Tag) at 2ug/ml (100uL/well) can bind CD155/PVR Protein, Rhesus, Recombinant (His Tag). The EC50 of CD155/PVR Protein, Rhesus, Recombinant (His Tag)is 200-900ng/mL.
2. Immobilized TIGIT Protein, Human, Recombinant (mFc Tag) at 2ug/ml (100uL/well) can bind CD155/PVR Protein, Rhesus, Recombinant (His Tag). The EC50 of CD155/PVR Protein, Rhesus, Recombinant (His Tag)is 200-900ng/mL.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
SDS-Page
SDS-Page
Testing Data
(Immobilized TIGIT Protein, Human, Recombinant (mFc Tag) at 2 ug/ml (100 ul/well) can bind CD155/PVR Protein, Rhesus, Recombinant (His Tag), the EC50 of CD155/PVR Protein, Rhesus, Recombinant (His Tag)is 200-900 ng/mL.)
Testing Data
(Immobilized TIGIT Protein, Human, Recombinant (mFc Tag) at 2 ug/ml (100 ul/well) can bind CD155/PVR Protein, Rhesus, Recombinant (His Tag), the EC50 of CD155/PVR Protein, Rhesus, Recombinant (His Tag)is 200-900 ng/mL.)
Related Product Information for PVR active protein
Background: CD155, commonly known as PVR (poliovirus receptor) and Necl-5 (nectin-like molecule-5), is a type I transmembrane single-span glycoprotein, and belongs to the nectins and nectin-like (Necl) subfamily. CD155 was originally identified based on its ability to mediate the cell attachment and entry of poliovirus (PV), an etiologic agent of the central nervous system disease poliomyelitis. The normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. CD155 may assist in an efficient humoral immune response generated within the intestinal immune system. It has been demonstrated that CD155 can be recognized and bond by DNAM-1 and CD96 which promote the adhesion, migration and NK-cell killing, and thus efficiently prime cell-mediated tumor-specific immunity.
Product Categories/Family for PVR active protein
References
Freistadt MS, et al. (2000) Hematopoietic cells from CD155-transgenic mice express CD155 and support poliovirus replication ex vivo. Microb Pathog. 29(4): 203-12.Sato T, et al. (2004) Involvement of heterophilic trans-interaction of Necl-5/Tage4/PVR/CD155 with nectin-3 in formation of nectin- and cadherin-based adherens junctions. Genes Cells. 9(9): 791-9.Kakunaga S, et al. (2004) Enhancement of serum- and platelet-derived growth factor-induced cell proliferation by Necl-5/Tage4/poliovirus receptor/CD155 through the Ras-Raf-MEK-ERK signaling. J Biol Chem. 279(35): 36419-25.Sato T, et al. (2005) Common signaling pathway is used by the trans-interaction of Necl-5/Tage4/PVR/CD155 and nectin, and of nectin and nectin during the formation of cell-cell adhesion. Cancer Sci. 96(9): 578-89.Minami Y, et al. (2007) Involvement of up-regulated Necl-5/Tage4/PVR/CD155 in the loss of contact inhibition in transformed NIH3T3 cells. Biochem Biophys Res Commun. 352(4): 856-60.
NCBI and Uniprot Product Information
Molecular Weight
The recombinant rhesus PVR consists of 327 amino acids and has a calculated molecular mass of 35.8kDa.
Protein Family
Similar Products
Product Notes
The PVR (Catalog #AAA8123519) is an Active Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Asn34 3. It is sometimes possible for the material contained within the vial of "CD155/PVR, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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