SDS-Page
(1ug loaded Tris-Bis PAGE under reducing condition. The purity is greater than 95%.)
COVID 19 Spike protein S Trimer Coronavirus Active Protein | COVID-19 active protein
Biotinylated SARS COV2 Spike protein S Trimer Recombinant
Purity
>95%, as determined by SDS-PAGE and HPLC
Synonyms
COVID 19 Spike protein S Trimer Coronavirus; Biotinylated SARS COV2 Spike protein S Trimer Recombinant; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike protein S Trimer; COVID-19 active protein
Host
HEK Cells
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant nCoV-2019 Spike S trimer protein was lyophilized from 0.2 um filtered PBS pH7.4.
Source Notes
DNA sequence encoding the COVID-19 Spike S protein extracellular domain,[16-1188]R683A (accession # QHD43416.1) fused to a a T4 Folsom trimerzation domain and a C-terminal His AVI tag was expressed in HEK Cells. The furin cleavage site has been mutated at position 683 and 685 so that the protein is not cleaved into the S1 and S2 primary domains by cellular proteases.
Biotinylation
The S trimer is biotinylated in vitro at a single site using the AVItagTM biotinylation technology.
Biological Activity
The activity was tested by binding Human ACE2 in functional ELISA assay (see figure).
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug(1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C
SDS-Page
(1ug loaded Tris-Bis PAGE under reducing condition. The purity is greater than 95%.)
SDS-Page
(1ug loaded Tris-Bis PAGE under reducing condition. The purity is greater than 95%.)
Activity
(Biotinylated COV2 S trimer(coating at 0.1ug-well) binding with Human ACE2-Fc (cat#RKQ9BYF1F) .The EC50 was determined to be 35 ng/ml)
Activity
(Biotinylated COV2 S trimer(coating at 0.1ug-well) binding with Human ACE2-Fc (cat#RKQ9BYF1F) .The EC50 was determined to be 35 ng/ml)
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
Recombinant 2019-nCoV Spike S trimer protein is a protein consisting of the S1+S2 subunits amino acid residues. The protein has a theoretical MW of 140kDa,however due to glycosylation migrates as an approximately 200 kDa protein on reduced SDS-PAGE.
Similar Products
Product Notes
The COVID-19 (Catalog #AAA553736) is an Active Protein produced from HEK Cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "COVID 19 Spike protein S Trimer Coronavirus, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
