Human Histone deacetylase 8 ELISA Kit | HDAC8 elisa kit

Human Histone deacetylase 8 ELISA Kit

Cat. Num. AAA765934

• Gene Names: HDAC8; HD8; WTS; RPD3; CDA07; CDLS5; MRXS6; HDACL1
• Reactivity: Human
• Synonyms: Histone deacetylase 8; Human Histone deacetylase 8 ELISA Kit; HDAC8 (Histone deacetylase 8)/CDA07/HDACL1/RPD3; HDAC8 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of HDAC. No significant cross-reactivity or interference between HDAC and analogues was observed.
• Sequence Length: 377

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: <0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HDAC were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HDAC were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HDAC8 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-HDAC antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-HDAC antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the HDAC amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of HDAC can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 7710996
• NCBI GeneID: 55869
• UniProt Accession #: Q9BY41; Q86VC8; Q9NP76; Q9NYH4; A6ND12; A6ND61; A6NET3; A6NJR3; A8MQ62; B4DKN0; B4DV22
• Molecular Weight: 16,050 Da
• NCBI Official Full Name: histone deacetylase 8
• NCBI Official Synonym Full Names: histone deacetylase 8
• NCBI Official Symbol: HDAC8
• NCBI Official Synonym Symbols: HD8; WTS; RPD3; CDA07; CDLS5; MRXS6; HDACL1
• NCBI Protein Information: histone deacetylase 8
• UniProt Protein Name: Histone deacetylase 8
• Protein Family: Histone deacetylase
• UniProt Gene Name: HDAC8
• UniProt Synonym Gene Names: HDACL1; HD8
• UniProt Entry Name: HDAC8_HUMAN

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

HDAC8: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility. Belongs to the histone deacetylase family. HD type 1 subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.5.1.98; Hydrolase

Chromosomal Location of Human Ortholog: Xq13

Cellular Component: cytoplasm; cytosol; histone deacetylase complex; nuclear chromosome; nucleoplasm; nucleus; plasma membrane

Molecular Function: histone deacetylase activity; Hsp70 protein binding; Hsp90 protein binding; metal ion binding; NAD-dependent histone deacetylase activity (H3-K14 specific); protein binding; transcription factor binding

Biological Process: chromatin assembly or disassembly; chromatin modification; establishment and/or maintenance of chromatin architecture; mitotic cell cycle; negative regulation of protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter; regulation of protein stability; regulation of telomere maintenance; sister chromatid cohesion; transcription, DNA-dependent

Disease: Wilson-turner X-linked Mental Retardation Syndrome