Histone Deacetylase 4 Recombinant Protein | HDAC4 recombinant protein

Recombinant Histone Deacetylase 4 (HDAC4)

Cat. Num. AAA2031436

• Gene Names: HDAC4; HD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Histone Deacetylase 4; Recombinant Histone Deacetylase 4 (HDAC4); HDAC4 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; RTVVMPI ANEFAPDVVL VSSGFDAVEG HPTPLGGYNL SAKCFGYLTKQLMGLAGGRL VLALEGGHDL TAICDASEAC VSALLGNELE PLPEKVLHQRPNANAVHSME KVMDIHSKYW RCLQRLSSTV GHSLIEAQKC EKEEAETVTAMASLSVGVKP AEKRSEEEPM EEEPPL
• Sequence Length: 1084

• Applicable Applications for HDAC4 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 22.4kDa
• Accurate Molecular Mass (KD): 22kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and S-tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Arg904~Leu1076
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 153085395
• NCBI GeneID: 9759
• NCBI Accession #: NP_006028.2
• NCBI GenBank Nucleotide #: NM_006037.3
• Molecular Weight: 106,367 Da
• NCBI Official Full Name: histone deacetylase 4
• NCBI Official Synonym Full Names: histone deacetylase 4
• NCBI Official Symbol: HDAC4
• NCBI Official Synonym Symbols: HD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A
• NCBI Protein Information: histone deacetylase 4
• UniProt Protein Name: Histone deacetylase 4
• Protein Family: Histone deacetylase
• UniProt Gene Name: HDAC4
• UniProt Synonym Gene Names: KIAA0288; HD4
• UniProt Entry Name: HDAC4_HUMAN

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008]

Uniprot Description

HDAC4: a transcriptional regulator of the histone deacetylase family, subfamily 2. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events. Does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3.

Protein type: EC 3.5.1.98; Nuclear receptor co-regulator; Hydrolase; Deacetylase

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: nucleoplasm; transcriptional repressor complex; histone deacetylase complex; cytoplasm; actomyosin; cytosol; neuromuscular junction; nucleus; Z disc; A band

Molecular Function: potassium ion binding; NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; transcription activator binding; zinc ion binding; NAD-dependent histone deacetylase activity (H3-K14 specific); sequence-specific DNA binding; histone deacetylase binding; protein deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription corepressor activity; protein kinase binding; transcription factor binding

Biological Process: cardiac muscle hypertrophy; positive regulation of transcription, DNA-dependent; response to denervation involved in regulation of muscle adaptation; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; negative regulation of cell proliferation; positive regulation of cell proliferation; negative regulation of osteoblast differentiation; inflammatory response; negative regulation of glycolysis; skeletal development; response to drug; nervous system development; regulation of skeletal muscle fiber development; transcription, DNA-dependent; B cell activation; negative regulation of transcription factor activity; histone deacetylation; osteoblast development; positive regulation of protein sumoylation; chromatin remodeling; B cell differentiation; regulation of protein binding; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent