HBeAg recombinant protein
HBeAg Protein
Applications
ELISA
Purity
>90% by SDS-PAGE
Synonyms
HBeAg; HBeAg Protein; Hepatitis BeAg; HBeAg recombinant protein
Host
E. coli
Purity/Purification
>90% by SDS-PAGE
Form/Format
Liquid; Each vial contains 0.02 M sodium phosphate (pH7.2) with 0.02% sodium azide.
Concentration
4.5 mg/ml (varies by lot)
Applicable Applications for HBeAg recombinant protein
ELISA
Application Notes
Other applications have not been tested. The optimal dilutions should be determined by end user.
Inactivation
Heated for 10 hours at 60°C before ultracentrifugation.
Preparation and Storage
Ship at 4 degree C. Upon receipt, aliquot and store at -20 degree C or -80 degree C for long term. Avoid repeated freeze and thaw cycles.
Related Product Information for HBeAg recombinant protein
Description: Recombinant full length human Hepatitis BeAg protein.
Background: HBeAg is the extracellular form of HBcAg (Hepatitis B viral protein, core antigen), hence why the presence of both are markers of viral replication, and antibodies to these antigens are markers of a decline in replication. Multiple protein products can be produced from the same DNA sequence. When "ORF Core" and "Pre C" are translated together, the result is "HBeAg". Both HBeAg and HBcAg are made from the same reading frame, but HBeAg is secreted from cells and accumulates in serum as an immunologically distinct soluble antigen. HBeAg is secreted and found in the serum of patients and serves as a marker of active replication in chronic hepatitis.
Background: HBeAg is the extracellular form of HBcAg (Hepatitis B viral protein, core antigen), hence why the presence of both are markers of viral replication, and antibodies to these antigens are markers of a decline in replication. Multiple protein products can be produced from the same DNA sequence. When "ORF Core" and "Pre C" are translated together, the result is "HBeAg". Both HBeAg and HBcAg are made from the same reading frame, but HBeAg is secreted from cells and accumulates in serum as an immunologically distinct soluble antigen. HBeAg is secreted and found in the serum of patients and serves as a marker of active replication in chronic hepatitis.
References
1. Kimura T, Rokuhara A, Matsumoto A, et al. (May 2003). "New enzyme immunoassay for detection of hepatitis B virus core antigen (HBcAg) and relation between levels of HBcAg and HBV DNA". J. Clin. Microbiol. 41 (5): 1901-6.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
HBeAg
Uniprot Description
May regulate immune response to the intracellular capsid in acting as a T-cell tolerogen, by having an immunoregulatory effect which prevents destruction of infected cells by cytotoxic T-cells.
Similar Products
Product Notes
The HBeAg (Catalog #AAA355623) is a Recombinant Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HBeAg can be used in a range of immunoassay formats including, but not limited to, ELISA. Other applications have not been tested. The optimal dilutions should be determined by end user. Researchers should empirically determine the suitability of the HBeAg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HBeAg, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
