Typical Testing Data/Standard Curve (for reference only)
TIM-1/KIM-1 ELISA Pair Set | HAVCR1 elisa pair set
TIM-1/KIM-1 Matched ELISA Antibody Pair Set, Mouse
Synonyms
TIM-1/KIM-1; TIM-1/KIM-1 Matched ELISA Antibody Pair Set; Mouse; Mouse TIM-1/KIM-1/HACVR ELISA Pair Set; hepatitis A virus cellular receptor 1; AI503787 Matched ELISA Antibody Pair Set; KIM-1 Matched ELISA Antibody Pair Set; TIM-1 Matched ELISA Antibody Pair Set; Tim1 Matched ELISA Antibody Pair Set; Timd1 Matched ELISA Antibody Pair Set; HAVCR1 elisa pair set
Assay Type
Quantitative Sandwich
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for HAVCR1 elisa pair set
Intended Uses: The Mouse TIM-1 / KIM-1 / HACVR ELISA Pair Set is for the quantitative determination of Mouse TIM-1 / KIM-1 / HACVR. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
Principle of the Assay: The Mouse TIM-1 / KIM-1 / HACVR ELISA Pair Set is for the quantitative determination of Mouse TIM-1 / KIM-1 / HACVR. The.ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Mouse TIM-1 / KIM-1 / HACVR coated on a 96-well plate. Standards and samples are added to the wells, and any Mouse TIM-1 / KIM-1 / HACVR present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Mouse TIM-1 / KIM-1 / HACVR polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Mouse TIM-1 / KIM-1 / HACVR present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
Background/Introduction: Human HAV cellular receptor 1 (HAVCR1), also known as Kidney injury molecule 1 (KIM-1) and T cell immunoglobulinmucin 1 (TIM-1), is a type â… integral membrane glycoprotein. KIM-1 protein is widely expressed with highest levels in kidney and testis. It has been shown to play a major role as a human susceptibility gene for asthma, allergy and autoimmunity. IgA1lambda is a specific ligand of KIM-1 protein and that their association has a synergistic effect in virus-receptor interactions. KIM-1 involves in the pathogenesis of acute kidney injury. It had been confirmed that KIM-1 is a human urinary renal dysfunction biomarker. Moreover, KIM-1 protein is a novel regulatory molecule of flow-induced calcium signaling.
Principle of the Assay: The Mouse TIM-1 / KIM-1 / HACVR ELISA Pair Set is for the quantitative determination of Mouse TIM-1 / KIM-1 / HACVR. The.ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Mouse TIM-1 / KIM-1 / HACVR coated on a 96-well plate. Standards and samples are added to the wells, and any Mouse TIM-1 / KIM-1 / HACVR present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Mouse TIM-1 / KIM-1 / HACVR polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Mouse TIM-1 / KIM-1 / HACVR present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
Background/Introduction: Human HAV cellular receptor 1 (HAVCR1), also known as Kidney injury molecule 1 (KIM-1) and T cell immunoglobulinmucin 1 (TIM-1), is a type â… integral membrane glycoprotein. KIM-1 protein is widely expressed with highest levels in kidney and testis. It has been shown to play a major role as a human susceptibility gene for asthma, allergy and autoimmunity. IgA1lambda is a specific ligand of KIM-1 protein and that their association has a synergistic effect in virus-receptor interactions. KIM-1 involves in the pathogenesis of acute kidney injury. It had been confirmed that KIM-1 is a human urinary renal dysfunction biomarker. Moreover, KIM-1 protein is a novel regulatory molecule of flow-induced calcium signaling.
References
Tami C, et al. (2007) Immunoglobulin A (IgA) is a natural ligand of hepatitis A virus cellular receptor 1 (HAVCR1), and the association of IgA with HAVCR1 enhances virus-receptor interactions. J Virol. 81(7): 3437-46.Rees AJ, et al. (2008) Kim-1/Tim-1: from biomarker to therapeutic target? Nephrol Dial Transplant. 23(11): 3394-6.Chaturvedi S, et al. (2009) Assay validation for KIM-1: human urinary renal dysfunction biomarker. Int J Biol Sci. 5(2): 128-34.
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Product Notes
The HAVCR1 (Catalog #AAA8118490) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "TIM-1/KIM-1, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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