Testing Data
TIM-1/KIM-1, Canine ELISA Pair Set | HAVCR1 elisa pair set
TIM-1/KIM-1 Matched ELISA Antibody Pair Set, Canine
Synonyms
TIM-1/KIM-1; Canine; TIM-1/KIM-1 Matched ELISA Antibody Pair Set; Canine KIM-1/TIM1/HAVCR1 ELISA Pair Set; hepatitis A virus cellular receptor 1; HAVCR1 elisa pair set
Specificity
Quantitative determination of Canine TIM-1/KIM-1
Assay Type
Solid Phase Sandwich ELISA
Detection Range
125-8000pg/mL
Sensitivity
125pg/ml
Materials Provided
1. Capture Antibody 1 mg/mL of rabbit anti-Canine TIMD1 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating.
2. Detection Antibody 1 mg/mL rabbit anti-Canine TIMD1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % glycerol, pH 7.4). Dilute to working concentration of 1ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 500 ng of recombinant Canine TIMD1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 8ng/mL is recommended.
2. Detection Antibody 1 mg/mL rabbit anti-Canine TIMD1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % glycerol, pH 7.4). Dilute to working concentration of 1ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 500 ng of recombinant Canine TIMD1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 8ng/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for HAVCR1 elisa pair set
HAV cellular receptor 1 (HAVCR1), also known as Kidney injury molecule 1 (KIM-1) and T cell immunoglobulin mucin 1 (TIM-1), is a type of integral membrane glycoprotein. KIM-1 protein is widely expressed with the highest levels in the kidney and testis. It has been shown to play a major role as a human susceptibility gene for asthma, allergy, and autoimmunity. IgA1lambda is a specific ligand of KIM-1 protein and that their association has a synergistic effect in virus-receptor interactions. KIM-1 involves in the pathogenesis of acute kidney injury. It had been confirmed that KIM-1 is a human urinary renal dysfunction biomarker. Moreover, KIM-1 protein is a novel regulatory molecule of flow-induced calcium signaling.
This TIM-1/KIM-1 Matched ELISA Antibody Pair Set, Canine is a solid phase sandwich ELISA for quantitative determination of Canine TIM-1/KIM-1. It contains Canine TIM-1/KIM-1 capture antibody, Canine TIM-1/KIM-1 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Canine TIM-1/KIM-1 protein.
This TIM-1/KIM-1 Matched ELISA Antibody Pair Set, Canine is a solid phase sandwich ELISA for quantitative determination of Canine TIM-1/KIM-1. It contains Canine TIM-1/KIM-1 capture antibody, Canine TIM-1/KIM-1 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Canine TIM-1/KIM-1 protein.
References
Tami C, et al. (2007) Immunoglobulin A (IgA) is a natural ligand of hepatitis A virus cellular receptor 1 (HAVCR1), and the association of IgA with HAVCR1 enhances virus-receptor interactions. J Virol. 81(7): 3437-46.Rees AJ, et al. (2008) Kim-1/Tim-1: from biomarker to therapeutic target? Nephrol Dial Transplant. 23(11): 3394-6.Chaturvedi S, et al. (2009) Assay validation for KIM-1: human urinary renal dysfunction biomarker. Int J Biol Sci. 5(2): 128-34.
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Product Notes
The HAVCR1 (Catalog #AAA8118528) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "TIM-1/KIM-1, Canine, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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