CXCL14 elisa kit

Chinese Hamster CXCL14 PicoKine ELISA Kit

Cat. Num. AAA1751106

• Gene Names: CXCL14; KEC; KS1; BMAC; BRAK; NJAC; MIP2G; MIP-2g; SCYB14
• Reactivity: Hamster; There is no detectable cross-reactivity with other relevant proteins.
• Applications: ELISA
• Synonyms: CXCL14; Chinese Hamster CXCL14 PicoKine ELISA Kit; C-X-C motif chemokine 14; Chemokine BRAK; MIP-2G; Small-inducible cytokine B14; MIP2G; NJAC; SCYB14; PSEC0212; UNQ240/PRO273; CXCL14 elisa kit

• Reactivity: Hamster; There is no detectable cross-reactivity with other relevant proteins.
• Specificity: Natural and recombinant 215 Chinese Hamster CXCL14
• Sequence Length: 111

• Applicable Applications for CXCL14 elisa kit: ELISA (EIA)
• Assay Type: Quantitative Sandwich
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CXCL14 elisa kit

Principle of the Assay: 215 Chinese Hamster CXCL14 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Chinese Hamster CXCL14 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli; Immunogen sequence: S35-E111) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Chinese Hamster CXCL14 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the 215 Chinese Hamster CXCL14 amount of sample captured in plate.

Background: Chemokine(C-X-C motif) ligand 14(CXCL14) is a small cytokine belonging to the CXC chemokine family that is also known as BRAK(for breast and kidney-expressed chemokine). This gene is mapped to 5q31.1. CXCL14 is constitutively expressed at high levels in many normal tissues, it has been found that fibroblasts are the primary source of CXCL14 and that CXCL14 is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation. It is also a potent chemoattractant and activator of dendritic cells, is implicated in homing of these cells, and can stimulate the migration of activated NK cells. CXCL14 also inhibits angiogenesis, possibly as a result of its ability to block endothelial cell chemotaxis. In addition to it, CXCL14 is a potent chemokine for immature DCs, with a chemoattractive activity comparable to bacterial fMLP and MIP1A.

Product Categories/Family for CXCL14 elisa kit

Immunology; Innate Immunity; Chemokines; Alpha Chemokines (CXC); Cancer; Oncoproteins/Suppressors; Tumor Suppressors

NCBI and Uniprot Product Information

• NCBI GI #: 20149565
• NCBI GeneID: 9547
• NCBI Accession #: NP_004878.2
• NCBI GenBank Nucleotide #: NM_004887.4
• UniProt Accession #: O95715; Q6UW97; Q86U69; Q9BTR1; Q9NS21; B3KQU8
• Molecular Weight: 13078 MW
• NCBI Official Full Name: C-X-C motif chemokine 14
• NCBI Official Synonym Full Names: C-X-C motif chemokine ligand 14
• NCBI Official Symbol: CXCL14
• NCBI Official Synonym Symbols: KEC; KS1; BMAC; BRAK; NJAC; MIP2G; MIP-2g; SCYB14
• NCBI Protein Information: C-X-C motif chemokine 14
• UniProt Protein Name: C-X-C motif chemokine 14
• Protein Family: C-X-C motif chemokine
• UniProt Gene Name: CXCL14
• UniProt Synonym Gene Names: MIP2G; NJAC; SCYB14

NCBI Description

This antimicrobial gene belongs to the cytokine gene family which encode secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC (Cys-X-Cys) subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes but not for lymphocytes, dendritic cells, neutrophils or macrophages. It has been implicated that this cytokine is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation. [provided by RefSeq, Sep 2014]

Uniprot Description

Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactic for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays.