CXCL14 (BRAK) Recombinant Protein | CXCL14 recombinant protein

CXCL14 (BRAK)

Cat. Num. AAA517297

• Gene Names: CXCL14; KEC; KS1; BMAC; BRAK; NJAC; MIP2G; MIP-2g; SCYB14
• Applications: Cell Assay
• Synonyms: CXCL14 (BRAK); Chemokine BRAK; MIP-2G; Small-inducible cytokine B14.; CXCL14 recombinant protein

• Host: E Coli
• Form/Format: Lyophilized from a 0.2 um filtered solution in PBS

• Applicable Applications for CXCL14 recombinant protein: Cell Assay
• Species: Human
• Endotoxin Level: <1.0EU/ug of recombinant protein as determined by the LAL method.
• Sample Cell Assay Data: The ED50 is 0.25-1.25ng/mL as determined by its ability to induce calcium flux in activated monocytes.
• Reconstitution: Reconstitute in sterile 4mM HCl at 100ug/mL (containing at least 0.1% human or bovine serum albumin).
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at -70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

SDS-Page

(Purity: Recombinant Human CXCL14 (BRAK) resolved on a 17% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250.)

Related Product Information for CXCL14 recombinant protein

Recombinant Human CXCL14 (BRAK) was expressed in E Coli cells. The protein accession number is O95715
Chemokine (CXC motif) ligand 14, also known as breast and kidney-expressed chemokine (BRAK), is a CXC chemokine that is a potent chemoattractant for neutrophils but not for T-cells, B-cells, monocytes or granulocytes. Mature CXCL14 has many conserved features of the CXC family and is 30% homologous to the MIP-alpha and MIP-beta sequences, but differs in that it has an additional five amino acid sequence in between the third and fourth cysteines and a short N-terminus. It is usually highly expressed near its fibroblast source and in epidermal fibroblasts and keratinocytes of skin, but is absent from cancer cells.

Product Categories/Family for CXCL14 recombinant protein

Growth Factors; Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 20149565
• NCBI GeneID: 9547
• NCBI Accession #: NP_004878.2
• NCBI GenBank Nucleotide #: NM_004887.4
• UniProt Accession #: O95715; Q6UW97; Q86U69; Q9BTR1; Q9NS21; B3KQU8
• Molecular Weight: 13,078 Da
• NCBI Official Full Name: C-X-C motif chemokine 14
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 14
• NCBI Official Symbol: CXCL14
• NCBI Official Synonym Symbols: KEC; KS1; BMAC; BRAK; NJAC; MIP2G; MIP-2g; SCYB14
• NCBI Protein Information: C-X-C motif chemokine 14; bolekine; MIP-2 gamma; chemokine BRAK; breast and kidney; tumor-suppressing chemokine; small-inducible cytokine B14; CXC chemokine in breast and kidney; small inducible cytokine subfamily B (Cys-X-Cys), member 14 (BRAK)
• UniProt Protein Name: C-X-C motif chemokine 14
• Protein Family: C-X-C motif chemokine
• UniProt Gene Name: CXCL14
• UniProt Synonym Gene Names: MIP2G; NJAC; SCYB14
• UniProt Entry Name: CXL14_HUMAN

NCBI Description

This gene belongs to the cytokine gene family which encode secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC (Cys-X-Cys) subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes but not for lymphocytes, dendritic cells, neutrophils or macrophages. It has been implicated that this cytokine is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation. [provided by RefSeq, Jul 2008]

Uniprot Description

CXCL14: Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactic for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Chemokine; Secreted

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: Golgi apparatus; extracellular space

Molecular Function: chemokine activity

Biological Process: cell-cell signaling; immune response; signal transduction; negative regulation of myoblast differentiation; chemotaxis; inner ear development