HAAO recombinant protein

HAAO Protein, Rat, Recombinant (His Tag)

Cat. Num. AAA8123488

• Purity: >90% as determined by SDS-PAGE
• Synonyms: HAAO; HAAO Protein; Rat; Recombinant (His Tag); Rat HAAO/3-HAO Protein (His Tag); 3-hydroxyanthranilate 3; 4-dioxygenase; HAAO recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly286

• Species: Rat
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the rat Haao (NP_064461.1)(Met1-Gly286) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 9910256
• NCBI GeneID: 56823
• NCBI Accession #: NP_064461.1
• NCBI GenBank Nucleotide #: NM_020076.2
• UniProt Accession #: P46953; P70474; Q5RKK0; Q64556
• Molecular Weight: 32,582 Da
• NCBI Official Full Name: 3-hydroxyanthranilate 3,4-dioxygenase
• NCBI Official Synonym Full Names: 3-hydroxyanthranilate 3,4-dioxygenase
• NCBI Official Symbol: Haao
• NCBI Protein Information: 3-hydroxyanthranilate 3,4-dioxygenase
• UniProt Protein Name: 3-hydroxyanthranilate 3,4-dioxygenase
• UniProt Gene Name: Haao
• UniProt Synonym Gene Names: 3-HAO; HAD

NCBI Description

enzyme responsible for the synthesis of quinolinic acid [RGD, Feb 2006]

Uniprot Description

HAAO: Catalyzes the oxidative ring opening of 3- hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate. Belongs to the 3-HAO family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - tryptophan; EC 1.13.11.6; Oxidoreductase

Chromosomal Location of Human Ortholog: 6q12

Cellular Component: cytoplasm; cytosol; mitochondrial membrane

Molecular Function: 3-hydroxyanthranilate 3,4-dioxygenase activity; ferrous iron binding; iron ion binding; oxygen binding

Biological Process: anthranilate metabolic process; NAD biosynthetic process; quinolinate biosynthetic process; quinolinate metabolic process; response to cadmium ion; response to zinc ion; tryptophan catabolic process