Granzyme H Recombinant Protein | GZMH recombinant protein

Recombinant Human Granzyme H Protein (His tag)

Cat. Num. AAA2546311

• Gene Names: GZMH; CCP-X; CGL-2; CSP-C; CTLA1; CTSGL2
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: Granzyme H; Recombinant Human Granzyme H Protein (His tag); GZMH; CCP-X; CGL-2; CSP-C; CTLA1; CTSGL2; GZMH recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 246

• Application Notes: The secreted recombinant human Granzyme H comprises 239 amino acids with a predicted molecular mass of 26.7 kDa. As a result of glycosylation, rh Granzyme H migrates as an approximately 36 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Glu 19
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for GZMH recombinant protein

Background: Granzymes are key components of the immune response that play important roles in eliminating host cells infected by intracellular pathogens. Several granzymes are potent inducers of cell death. A total of eight granzymes (A-G and M) have been identified in the mouse, but only five are known in humans (A, B, H, M and granzyme 3), and granzyme H appears to be specifically human. Human granzyme H is a neutral serine protease that is expressed predominantly in the lymphokine-activated killer (LAK)/natural killer (NK) compartment of the immune system. In adenovirus-infected cells in which granzyme B (gzmB) and downstream apoptosis pathways are inhibited, granzyme H directly cleaves the adenovirus DNA-binding protein (DBP), a viral component absolutely required for viral DNA replication. This virus demonstrated that gzmH directly induces an important decay in viral DNA replication. Interestingly, gzmH also cleaves the adenovirus 100K assembly protein, a major inhibitor of gzmB, and relieves gzmB inhibition. Granzyme H has a very high amino acid identity (>90%) with many portions of the granzyme B sequence, particularly near the amino terminus of the molecule despite performing a distinct enzymic function.

Description: A DNA sequence encoding the human Granzyme H (NP_219491.1) (Met 1-Leu 246) with a C-terminal polyhistidine tag was expressed.

NCBI and Uniprot Product Information

• NCBI GI #: 15529990
• NCBI GeneID: 2999
• NCBI Accession #: NP_219491.1
• NCBI GenBank Nucleotide #: NM_033423.4
• UniProt Accession #: P20718; Q6XGZ0; Q6XGZ1; G3V2C5
• Molecular Weight: 12,609 Da
• NCBI Official Full Name: granzyme H isoform 1 preproprotein
• NCBI Official Synonym Full Names: granzyme H
• NCBI Official Symbol: GZMH
• NCBI Official Synonym Symbols: CCP-X; CGL-2; CSP-C; CTLA1; CTSGL2
• NCBI Protein Information: granzyme H
• UniProt Protein Name: Granzyme H
• Protein Family: Granzyme
• UniProt Gene Name: GZMH
• UniProt Synonym Gene Names: CGL2; CTSGL2; CTSGL2; CSP-C

NCBI Description

This gene encodes a member of the peptidase S1 family of serine proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate a chymotrypsin-like protease. This protein is reported to be constitutively expressed in the NK (natural killer) cells of the immune system and may play a role in the cytotoxic arm of the innate immune response by inducing target cell death and by directly cleaving substrates in pathogen-infected cells. This gene is present in a gene cluster with another member of the granzyme subfamily on chromosome 14. [provided by RefSeq, Nov 2015]

Uniprot Description

GZMF: Cytotoxic chymotrypsin-like serine protease with preference for bulky and aromatic residues at the P1 position and acidic residues at the P3' and P4' sites. Probably necessary for target cell lysis in cell-mediated immune responses. Belongs to the peptidase S1 family. Granzyme subfamily.

Protein type: EC 3.4.21.-; Protease

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: cytoplasm; intracellular membrane-bound organelle; membrane

Molecular Function: serine-type endopeptidase activity

Biological Process: immune response; protein processing