Guinea Pig PPARgammaC1alpha ELISA Kit | PPARgammaC1alpha elisa kit
Guinea pig PPARgammaC1alpha (Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha) ELISA Kit
Reactivity
Guinea Pig
Synonyms
PPARgammaC1alpha; Guinea pig PPARgammaC1alpha (Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha) ELISA Kit; PPARgammaC1alpha elisa kit
Reactivity
Guinea Pig
Specificity
This kit recognizes natural and recombinant Guinea pig PPARgammaC1alpha. No significant cross-reactivity or interference between Guinea pig PPARgammaC1alpha and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
31.25-2000pg/mL
Sensitivity
Min: 18.75pg/mL; Max: 2000pg/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PPARgammaC1alpha elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Guinea pig PPARgammaC1alpha concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PPARgammaC1alpha. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PPARgammaC1alpha and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PPARgammaC1alpha, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PPARgammaC1alpha. You can calculate the concentration of PPARgammaC1alpha in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PPARgammaC1alpha. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PPARgammaC1alpha and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PPARgammaC1alpha, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PPARgammaC1alpha. You can calculate the concentration of PPARgammaC1alpha in the samples by comparing the OD of the samples to the standard curve.
