Guinea Pig CYTIP ELISA Kit | CYTIP elisa kit
Guinea pig CYTIP (Cytohesin Interacting Protein) ELISA Kit
Gene Names
CYTIP; HE; B3-1; CASP; CYBR; CYTHIP; PSCDBP
Reactivity
Guinea Pig
Synonyms
CYTIP; Guinea pig CYTIP (Cytohesin Interacting Protein) ELISA Kit; CYTIP elisa kit
Reactivity
Guinea Pig
Specificity
This kit recognizes natural and recombinant Guinea pig CYTIP. No significant cross-reactivity or interference between Guinea pig CYTIP and analogues was observed.
Sequence Length
359
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.625-40ng/mL
Sensitivity
Min: 0.375ng/mL; Max: 40ng/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CYTIP elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Guinea pig CYTIP concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CYTIP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CYTIP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CYTIP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CYTIP. You can calculate the concentration of CYTIP in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CYTIP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CYTIP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CYTIP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CYTIP. You can calculate the concentration of CYTIP in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,172 Da
NCBI Official Full Name
cytohesin-interacting protein
NCBI Official Synonym Full Names
cytohesin 1 interacting protein
NCBI Official Symbol
CYTIP
NCBI Official Synonym Symbols
HE; B3-1; CASP; CYBR; CYTHIP; PSCDBP
NCBI Protein Information
cytohesin-interacting protein; cbp HE; cytohesin binder and regulator; cytohesin binding protein HE; cytohesin-1 interacting protein; cytohesin-associated scaffolding protein; cytohesin-binding protein HE; pleckstrin homology Sec7 and coiled-coil domains-binding protein; pleckstrin homology, Sec7 and coiled-coil domains, binding protein; pleckstrin homology, Sec7 and coiled/coil domains, binding protein
UniProt Protein Name
Cytohesin-interacting protein
Protein Family
UniProt Gene Name
CYTIP
UniProt Synonym Gene Names
PSCDBP; CYBR; CASP; Cbp HE
UniProt Entry Name
CYTIP_HUMAN
NCBI Description
The protein encoded by this gene contains 2 leucine zipper domains and a putative C-terminal nuclear targeting signal, but does not have any hydrophobic regions. This protein is expressed weakly in resting NK and T cells. The encoded protein modulates the activation of ARF genes by CYTH1. This protein interacts with CYTH1 and SNX27 proteins and may act to sequester CYTH1 protein in the cytoplasm.[provided by RefSeq, Aug 2008]
Uniprot Description
PSCDBP: By its binding to cytohesin-1 (CYTH1), it modifies activation of ARFs by CYTH1 and its precise function may be to sequester CYTH1 in the cytoplasm.
Protein type: Cell adhesion
Chromosomal Location of Human Ortholog: 2q11.2
Cellular Component: nucleoplasm; early endosome; cytoplasm; cell cortex
Molecular Function: protein binding
Biological Process: regulation of cell adhesion
