Guinea pig Alpha-1-Microglobulin/Bikunin Precursor (a1M) ELISA Kit | a1M elisa kit

Guinea pig Alpha-1-Microglobulin/Bikunin Precursor (a1M) ELISA Kit

Cat. Num. AAA2000251

• Gene Names: AMBP; A1M; HCP; ITI; UTI; EDC1; HI30; ITIL; IATIL; ITILC
• Reactivity: Guinea pig
• Synonyms: Alpha-1-Microglobulin/Bikunin Precursor (a1M); Guinea pig Alpha-1-Microglobulin/Bikunin Precursor (a1M) ELISA Kit; ELISA Kit for Alpha-1-Microglobulin/Bikunin Precursor (a1M); a1M elisa kit

• Reactivity: Guinea pig
• Specificity: This assay has high sensitivity and excellent specificity for detection of Alpha-1-Microglobulin/Bikunin Precursor (a1M).; No significant cross-reactivity or interference between Alpha-1-Microglobulin/Bikunin Precursor (a1M) and analogues was observed.
• Sequence Length: 352

• Samples: Serum, plasma, urine and other biological fluids
• Assay Type: Sandwich
• Detection Range: 0.781-50 ng/mL The standard curve concentrations used for the ELISA's were 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.125 ng/mL, 1.563 ng/mL, 0.781 ng/mL
• Sensitivity: Typically less than 0.52 ng/mL.
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-1-Microglobulin/Bikunin Precursor (a1M) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-1-Microglobulin/Bikunin Precursor (a1M) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all the reagents should be kept according to the label on each vial. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C while others should be at 4 degree C.

Related Product Information for a1M elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-Microglobulin/Bikunin Precursor (a1M). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-1-Microglobulin/Bikunin Precursor (a1M). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-1-Microglobulin/Bikunin Precursor (a1M), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-1-Microglobulin/Bikunin Precursor (a1M) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119607810
• NCBI GeneID: 259
• UniProt Accession #: P02760; P00977; P02759; P78491; Q2TU33; Q5TBD7; Q9UC58; Q9UDI8
• Molecular Weight: 38,999 Da
• NCBI Official Full Name: alpha-1-microglobulin/bikunin
• NCBI Official Synonym Full Names: alpha-1-microglobulin/bikunin precursor
• NCBI Official Symbol: AMBP
• NCBI Official Synonym Symbols: A1M; HCP; ITI; UTI; EDC1; HI30; ITIL; IATIL; ITILC
• NCBI Protein Information: protein AMBP; bikunin; uristatin; protein HC; trypstatin; uronic-acid-rich protein; growth-inhibiting protein 19; inter-alpha-trypsin inhibitor light chain; complex-forming glycoprotein heterogeneous in charge
• UniProt Protein Name: Protein AMBP
• Protein Family: Alpha-1-macroglobulin
• UniProt Gene Name: AMBP
• UniProt Synonym Gene Names: HCP; ITIL; Protein HC; ITI-LC
• UniProt Entry Name: AMBP_HUMAN

NCBI Description

This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes. [provided by RefSeq, Jul 2008]

Uniprot Description

AMBP: Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q32-q33

Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; plasma membrane; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protein homodimerization activity; IgA binding; heme binding; calcium oxalate binding; calcium channel inhibitor activity

Biological Process: negative regulation of JNK cascade; receptor-mediated endocytosis; viral reproduction; heme catabolic process; negative regulation of immune response; protein catabolic process; female pregnancy; cell adhesion; protein-chromophore linkage