GTF2B recombinant protein

GTF2B, 1-316aa, Human, His tag, E Coli

Cat. Num. AAA203455

• Gene Names: GTF2B; TF2B; TFIIB
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: GTF2B; 1-316aa; Human; His tag; E Coli; General transcription factor IIB; TF2B; TFIIB; General transcription factor IIB General transcription factor TFIIB; RNA polymerase II transcription factor IIB; S300 II; TF IIB; Transcription initiation factor IIB.; GTF2B recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1 M NaCl.
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MASTSRLDAL PRVTCPNHPD AILVEDYRAG DMICPECGLV VGDRVIDVGS EWRTFSNDKA TKDPSRVGDS QNPLLSDGDL STMIGKGTGA ASFDEFGNSK YQNRRTMSSS DRAMMNAFKE ITTMADRINL PRNIVDRTNN LFKQVYEQKS LKGRANDAIA SACLYIACRQ EGVPRTFKEI CAVSRISKKE IGRCFKLILK ALETSVDLIT TGDFMSRFCS NLCLPKQVQM AATHIARKAV ELDLVPGRSP ISVAAAAIYM ASQASAEKRT QKEIGDIAGV ADVTIRQSYR LIYPRAPDLF PTDFKFDTPV DKLPQL
• Sequence Length: 316

• Applicable Applications for GTF2B recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for GTF2B recombinant protein

GTF2B, also known as transcription factor II B, is one of several general transcription factors that make up the RNA polymerase II preinitiation complex. It localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. The protein serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. It is involved in the selection of the transcription start site. Recombinant GTF2B protein was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for GTF2B recombinant protein

Transcription & Translation

References

Ing N.H., et al. (1992) J. Biol. Chem. 267:17617-17623.; Bushnell, David A, et al. (2004) Science. 303 (5660):983-8.;

NCBI and Uniprot Product Information

• NCBI GI #: 4504193
• NCBI GeneID: 2959
• NCBI Accession #: NP_001505
• NCBI GenBank Nucleotide #: NM_001514.5
• UniProt Accession #: Q00403; Q5JS30; A8K1A7
• Molecular Weight: 36.9 kDa (336aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: transcription initiation factor IIB
• NCBI Official Synonym Full Names: general transcription factor IIB
• NCBI Official Symbol: GTF2B
• NCBI Official Synonym Symbols: TF2B; TFIIB
• NCBI Protein Information: transcription initiation factor IIB
• UniProt Protein Name: Transcription initiation factor IIB
• Protein Family: Transcription initiation factor
• UniProt Gene Name: GTF2B
• UniProt Synonym Gene Names: TF2B; TFIIB

NCBI Description

This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq, Jul 2008]

Uniprot Description

General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.