GST T2-2 recombinant protein
GST T2-2, Recombinant Human
Purity
>= 95% as assessed by inspection on a Coomassie Blue-stained SDS-PAGE gel.
Synonyms
GST T2-2; Recombinant Human; Recombinant Human GST T2-2; GST T2-2 recombinant protein
Host
Recombinant 6x His-tagged protein expressed in E Coli.
Specificity
Specific Activity: 12.6 Units/mg as assayed by the spectrophotometric determination of NADPH oxidation coupled to the glutathione peroxidase activity of GST T2-2 on cumene hydroperoxide (1.5 mM) in the presence of reduced glutathione (1 mM) in 100 mM NaPO4 (pH 7.0) at room temperature.
Purity/Purification
>= 95% as assessed by inspection on a Coomassie Blue-stained SDS-PAGE gel.
Concentration
1.91 mg/mL total protein using the Bradford protein assay with BSA as a standard. (varies by lot)
Storage Buffer
50 mM Tris-HCl (pH 7.5), 50 mM NaCl, 1 mM DTT, 1 mM EDTA and 50% glycerol.
Procedural Notes
This purified product exhibits moderate enzymatic activity for Cumene hydroperoxide, the substrate that is most commonly used to assess the glutathione peroxidase activity of soluble GSTs. However, as is suspected for GST activity analysis using synthetic and natural substrates, low concentrations (high dilutions) of the enzyme may result in lower activity values. In contrast, initial velocities are much higher for more concentrated levels of enzyme but the rate decreases rapidly. Therefore, activity towards GST substrates and these considerations provide guidance when assaying this product under low concentrations or when using substrates other than Cumene hydroperoxide.
Preparation and Storage
At -20 degree C; AVOID MULTIPLE FREEZE-THAW CYCLES.
References
Tan K.-L. and Board P.G., Biochem. J., 315:727-732 (1996)
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Product Notes
The GST T2-2 (Catalog #AAA480630) is a Recombinant Protein produced from Recombinant 6x His-tagged protein expressed in E Coli. and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "GST T2-2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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