Goat Glucose regulated protein 94 ELISA Kit | GRP94 elisa kit

Goat Glucose regulated protein 94 ELISA Kit

Cat. Num. AAA750393

• Gene Names: HSP90B1; TRA1; GRP94
• Reactivity: Goat
• Synonyms: Glucose regulated protein 94; Goat Glucose regulated protein 94 ELISA Kit; GRP94 elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This GRP94 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat GRP94. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Related Product Information for GRP94 elisa kit

Principle of the assay: GRP94 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GRP94 antibody and an GRP94-HRP conjugate. The assay sample and buffer are incubated together with GRP94-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GRP94 concentration since GRP94 from samples and GRP94-HRP conjugate compete for the anti-GRP94 antibody binding site. Since the number of sites is limited, as more sites are occupied by GRP94 from the sample, fewer sites are left to bind GRP94-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GRP94 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 403497
• NCBI GeneID: 404019
• Molecular Weight: 92,514 Da
• NCBI Official Full Name: GRP94
• NCBI Official Symbol: HSP90B1
• NCBI Official Synonym Symbols: TRA1; GRP94
• NCBI Protein Information: endoplasmin; GRP-94; HSP90 beta; tumor rejection antigen 1; 94 kDa glucose-regulated protein; tumor rejection antigen (gp96) 1; heat shock protein 90 kDa beta member 1
• UniProt Protein Name: Endoplasmin
• UniProt Gene Name: HSP90B1
• UniProt Synonym Gene Names: GRP94; TRA1; GRP-94
• UniProt Entry Name: ENPL_CANFA

Uniprot Description

GRP94: Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with AIMP1; regulates its retention in the endoplasmic reticulum. Interacts with OS9. Interacts with CNPY3. This interaction is disrupted in the presence of ATP. Interacts with TLR4 and TLR9, but not with TLR3. Belongs to the heat shock protein 90 family.

Protein type: Secreted; Secreted, signal peptide; Apoptosis; Heat shock protein

Cellular Component: extracellular matrix; endoplasmic reticulum membrane; focal adhesion; sarcoplasmic reticulum; endoplasmic reticulum lumen; perinuclear region of cytoplasm; plasma membrane; melanosome; midbody; nucleus; cytosol

Molecular Function: low-density lipoprotein receptor binding; RNA binding; unfolded protein binding; virion binding; protein phosphatase binding; ATP binding

Biological Process: actin rod formation; ER-associated protein catabolic process; protein folding; response to hypoxia; regulation of phosphoprotein phosphatase activity; negative regulation of apoptosis