Glutamate Receptor, Ionotropic, Kainate 2 Recombinant Protein | GRIK2 recombinant protein

Recombinant Glutamate Receptor, Ionotropic, Kainate 2 (GRIK2)

Cat. Num. AAA2033281

• Gene Names: GRIK2; EAA4; GLR6; MRT6; GLUK6; GLUR6; GluK2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Glutamate Receptor; Ionotropic; Kainate 2; Recombinant Glutamate Receptor; Kainate 2 (GRIK2); EAA4; GLR6; GLUR6; GluK2; Excitatory amino acid receptor 4; Glutamate receptor 6; GRIK2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: NTQVS SIIEKWSMER LQAPPKPDSG LLDGFMTTDA ALMYDAVHVV SVAVQQFPQM TVSSLQCNRH KPWRFGTRFM SLIKEAHWEG LTGRITFNKT NGLRTDFDLD VISLKEEGLE KIGTWDPASG LNMTESQKGK PANITDSLSN RSLIVTTILE EPYVLFKKSD KPLYGNDRFE GYCIDLLREL STILGFTYEI RLVEDGKYGA QDDANGQWNG MVRELIDHKA DLAVAPLAITYVREKVIDFS KPFMTLGISI LYRKPNGTNP GVFSFLNPLS P
• Sequence Length: 869

• Applicable Applications for GRIK2 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 34.7kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Asn286~Pro561
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 28559003
• NCBI GeneID: 2898
• NCBI Accession #: NP_786944.1
• NCBI GenBank Nucleotide #: NM_175768.3
• Molecular Weight: 77,112 Da
• NCBI Official Full Name: glutamate receptor ionotropic, kainate 2 isoform 2
• NCBI Official Synonym Full Names: glutamate ionotropic receptor kainate type subunit 2
• NCBI Official Symbol: GRIK2
• NCBI Official Synonym Symbols: EAA4; GLR6; MRT6; GLUK6; GLUR6; GluK2
• NCBI Protein Information: glutamate receptor ionotropic, kainate 2
• UniProt Protein Name: Glutamate receptor ionotropic, kainate 2
• Protein Family: Glutamate receptor ionotropic
• UniProt Gene Name: GRIK2
• UniProt Synonym Gene Names: GLUR6; GluK2; EAA4; GluR-6; GluR6
• UniProt Entry Name: GRIK2_HUMAN

NCBI Description

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing at multiple sites within the first and second transmembrane domains, which is thought to alter the structure and function of the receptor complex. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. Mutations in this gene have been associated with autosomal recessive mental retardation. [provided by RefSeq, Jul 2008]

Uniprot Description

GluR6: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus. Modulates cell surface expression of NETO2. Defects in GRIK2 are the cause of mental retardation autosomal recessive type 6 (MRT6). It is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, intellectual deficiency is the only primary symptom of non-syndromic mental retardation. MRT6 patients display mild to severe mental retardation and psychomotor development delay in early childhood. Patients do not have neurologic problems, congenital malformations, or facial dysmorphism. Body height, weight, and head circumference are normal. Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK2 subfamily. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Channel, calcium; Membrane protein, integral; Channel, ligand-gated

Chromosomal Location of Human Ortholog: 6q16.3

Cellular Component: cell junction; dendrite cytoplasm; integral to plasma membrane; kainate selective glutamate receptor complex; perikaryon; plasma membrane; postsynaptic density; postsynaptic membrane; presynaptic membrane; terminal button

Molecular Function: extracellular-glutamate-gated ion channel activity; kainate selective glutamate receptor activity; PDZ domain binding; protein homodimerization activity; ubiquitin conjugating enzyme binding; ubiquitin protein ligase binding

Biological Process: behavioral fear response; cellular calcium ion homeostasis; generation of action potential; glutamate signaling pathway; intracellular protein transport; ionotropic glutamate receptor signaling pathway; negative regulation of neuron apoptosis; negative regulation of synaptic transmission, glutamatergic; neuron apoptosis; positive regulation of neuron apoptosis; positive regulation of synaptic transmission; receptor clustering; regulation of excitatory postsynaptic membrane potential; regulation of inhibitory postsynaptic membrane potential; regulation of JNK cascade; regulation of long-term neuronal synaptic plasticity; regulation of short-term neuronal synaptic plasticity; regulation of synaptic transmission; synaptic transmission; synaptic transmission, glutamatergic; transport

Disease: Mental Retardation, Autosomal Recessive 6