Typical Testing Data/Standard Curve (for reference only)
Chicken GRIK2 ELISA Kit | GRIK2 elisa kit
Chicken GRIK2 (Glutamate Receptor, Ionotropic, Kainate 2) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GRIK2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GRIK2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GRIK2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GRIK2. You can calculate the concentration of GRIK2 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing at multiple sites within the first and second transmembrane domains, which is thought to alter the structure and function of the receptor complex. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. Mutations in this gene have been associated with autosomal recessive mental retardation. [provided by RefSeq, Jul 2008]
Uniprot Description
GluR6: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus. Modulates cell surface expression of NETO2. Defects in GRIK2 are the cause of mental retardation autosomal recessive type 6 (MRT6). It is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, intellectual deficiency is the only primary symptom of non-syndromic mental retardation. MRT6 patients display mild to severe mental retardation and psychomotor development delay in early childhood. Patients do not have neurologic problems, congenital malformations, or facial dysmorphism. Body height, weight, and head circumference are normal. Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK2 subfamily. 7 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Channel, ligand-gated; Channel, calcium; Membrane protein, integral
Chromosomal Location of Human Ortholog: 6q16.3
Cellular Component: dendrite cytoplasm; presynaptic membrane; postsynaptic membrane; kainate selective glutamate receptor complex; integral to plasma membrane; postsynaptic density; dendrite; plasma membrane; terminal button; perikaryon; cell junction
Molecular Function: protein homodimerization activity; extracellular-glutamate-gated ion channel activity; kainate selective glutamate receptor activity; ubiquitin conjugating enzyme binding; ubiquitin protein ligase binding; PDZ domain binding
Biological Process: regulation of synaptic transmission; regulation of long-term neuronal synaptic plasticity; synaptic transmission, glutamatergic; behavioral fear response; glutamate signaling pathway; generation of action potential; negative regulation of synaptic transmission, glutamatergic; regulation of short-term neuronal synaptic plasticity; regulation of JNK cascade; receptor clustering; positive regulation of synaptic transmission; cellular calcium ion homeostasis; synaptic transmission; intracellular protein transport; regulation of inhibitory postsynaptic membrane potential; neuron apoptosis; ionotropic glutamate receptor signaling pathway; transport; positive regulation of neuron apoptosis; negative regulation of neuron apoptosis; regulation of excitatory postsynaptic membrane potential
Disease: Mental Retardation, Autosomal Recessive 6
Research Articles on GRIK2
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Product Notes
The Chicken GRIK2 grik2 (Catalog #AAA2503019) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2503019 ELISA Kit recognizes Chicken GRIK2. It is sometimes possible for the material contained within the vial of "GRIK2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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