Gremlin 1 (GREM1) Recombinant Protein | GREM1 recombinant protein

Recombinant Gremlin 1 (GREM1)

Cat. Num. AAA2010530

• Gene Names: GREM1; DRM; PIG2; DAND2; IHG-2; GREMLIN; CKTSF1B1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Gremlin 1 (GREM1); Recombinant Gremlin 1 (GREM1); GREM1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.; MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSEF-KKGSQ GAIPPPDKAQ HNDSEQTQSP QQPGSRNRGR GQGRGTAMPG EEVLESSQEA LHVTERKYLK RDWCKTQPLK QTIHEEGCNS RTIINRFCYG QCNSFYIPRH IRKEEGSFQS CSFCKPKKFT TMMVTLNCPE LQPPTKKKRV TRVKQCRCIS IDLD
• Sequence Length: 143

• Applicable Applications for GREM1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Lys26~Asp184 (Accession # O60565) with two N-terminal Tags, His-tag and S-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 300797328
• NCBI GeneID: 26585
• NCBI Accession #: NP_001178252.1
• NCBI GenBank Nucleotide #: NM_001191323.1
• UniProt Accession #: O60565; Q52LV3; Q8N914; Q8N936
• Molecular Weight: 23.9kDa
• NCBI Official Full Name: gremlin-1 isoform 2
• NCBI Official Synonym Full Names: gremlin 1, DAN family BMP antagonist
• NCBI Official Symbol: GREM1
• NCBI Official Synonym Symbols: DRM; PIG2; DAND2; IHG-2; GREMLIN; CKTSF1B1
• NCBI Protein Information: gremlin-1; gremlin 1-like protein; DAN domain family member 2; increased in high glucose-2; cell proliferation-inducing gene 2 protein; cysteine knot superfamily 1, BMP antagonist 1; gremlin 1, cysteine knot superfamily, homolog; down-regulated in Mos-transformed cells protein
• UniProt Protein Name: Gremlin-1
• Protein Family: Gremlin
• UniProt Gene Name: GREM1
• UniProt Synonym Gene Names: CKTSF1B1; DAND2; DRM; IHG-2
• UniProt Entry Name: GREM1_HUMAN

NCBI Description

This gene encodes a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation. In mouse, this protein has been shown to relay the sonic hedgehog (SHH) signal from the polarizing region to the apical ectodermal ridge during limb bud outgrowth. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

GREM1: Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Down-regulates the BMP4 signaling in a dose-dependent manner. Acts as inhibitor of monocyte chemotaxis. Interacts with SLIT1 and SLIT2 in a glycosylation- dependent manner. By high glucose through TGFB1-mediated pathways in mesangial cell. Down-regulated in tumor cell lines. Highly expressed in small intestine, fetal brain and colon. Weakly expressed in brain, ovary, prostate, pancreas and skeletal muscle. In brain found in the region localized around the internal capsule in the large subcortical nuclei, including caudate, putamen, substantia nigra, thalamus and subthalamus. Predominantly expressed in normal cells including neurons, astrocytes and fibroblasts. Belongs to the DAN family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 15q13.3

Cellular Component: extracellular space; cell surface

Molecular Function: morphogen activity; protein binding; transmembrane receptor protein tyrosine kinase activator activity; vascular endothelial growth factor receptor 2 binding; cytokine activity; receptor agonist activity

Biological Process: limb development; collagen fibril organization; transmembrane receptor protein tyrosine kinase activation (dimerization); apoptosis; negative regulation of chondrocyte differentiation; positive regulation of receptor internalization; cell morphogenesis; positive regulation of NF-kappaB import into nucleus; signal transduction; activation of NF-kappaB transcription factor; negative regulation of BMP signaling pathway; negative regulation of bone mineralization; cell-cell signaling; positive regulation of cell proliferation; proximal/distal pattern formation; embryonic limb morphogenesis; determination of dorsal identity; positive regulation of telomerase activity; regulation of stress-activated MAPK cascade; positive regulation of angiogenesis; cell migration during sprouting angiogenesis; ureteric bud branching; regulation of focal adhesion formation; negative regulation of bone remodeling; negative regulation of osteoblast proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis