Granulocyte Colony Stimulating Factor, Recombinant, Human, Western Blot Positive Control Recombinant Protein
Granulocyte Colony Stimulating Factor, Recombinant, Human, Western Blot Positive Control (G-CSF, GCSF, C17orf33, Colony Stimulating Factor 3 (Granulocyte), CSF3, CSF3OS, Filgrastim, Lenograstim, MGC45931, Pluripoietin)
Applications
Western Blot
Purity
Purified (Highly Purified (~95%))
Synonyms
Granulocyte Colony Stimulating Factor; Recombinant; Human; Western Blot Positive Control; Western Blot Positive Control (G-CSF; GCSF; C17orf33; Colony Stimulating Factor 3 (Granulocyte); CSF3; CSF3OS; Filgrastim; Lenograstim; MGC45931; Pluripoietin); G-CSF; Pluripoietin; Western Blot Positive Control recombinant protein
Host
E Coli
Purity/Purification
Purified (Highly Purified (~95%))
Form/Format
Supplied as a liquid in SDS-PAGE sample buffer (reduced).
Applicable Applications for Granulocyte Colony Stimulating Factor, Recombinant, Human, Western Blot Positive Control recombinant protein
Western Blot
Application Notes
Suitable for use in Western Blot. Not suitable for use in ELISA or other applications where native protein is required.
Recommended Dilutions: Western Blot: 10ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels.
Recommended Dilutions: Western Blot: 10ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels.
Source Information
Recombinant corresponding to human GCSF, expressed in E.coli.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Temperature: Blue Ice
Shipping Temperature: Blue Ice
Related Product Information for Granulocyte Colony Stimulating Factor, Recombinant, Human, Western Blot Positive Control recombinant protein
GCSF (CSF3), is a glycoprotein that is produced and secreted by macrophages stimulated with endotoxin. It is also produced by a number of different tissues to stimulate the bone marrow to produce granulocytes and stem cells. G-CSF then stimulates the bone marrow to pulse them out of the marrow into the blood. It also stimulates the survival, proliferation, differentiation, and function of neutrophil precursors and mature neutrophils. The natural human GCSF exists in two forms, a 174- and 180-amino-acid-long protein (precursor 204aa, mature peptide 175aa; chromosome 17q11.2-q12, ~19.6kD). The more-abundant and more-active 174aa form has been used in the development of pharmaceutical products (Available under the names Neupogen or Granulokine (Amgen/Roche) and Granocyte (Rhone-Poulenc). GCSF is used to treat neutropenia (a disorder characterized by an extremely low number of neutrophils in blood). The PEG (polyethylene glycol) form has a much longer half-life, reducing the necessity of daily injections. G-CSF stimulates the production of white blood cells. Recombinant G-CSF is used in certain cancer patients to accelerate recovery from neutropenia after chemotherapy, allowing higher-intensity treatment regimens. G-CSF is also used to increase the number of hematopoietic stem cells in the blood before collection by leukapheresis for use in hematopoietic stem cell transplantation.
GCSF mRNA is alternatively spliced to produce 2 isoforms. Isoform 1 (mature peptide 175aa or long form) and isoform 2 (short form, missing 66-68aa). Both forms have authentic GCSF activity.
GCSF mRNA is alternatively spliced to produce 2 isoforms. Isoform 1 (mature peptide 175aa or long form) and isoform 2 (short form, missing 66-68aa). Both forms have authentic GCSF activity.
Product Categories/Family for Granulocyte Colony Stimulating Factor, Recombinant, Human, Western Blot Positive Control recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
granulocyte colony stimulating factor
Similar Products
Product Notes
The Granulocyte Colony Stimulating Factor, Recombinant, Human, Western Blot Positive Control (Catalog #AAA650565) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Granulocyte Colony Stimulating Factor, Recombinant, Human, Western Blot Positive Control can be used in a range of immunoassay formats including, but not limited to, Western Blot. Suitable for use in Western Blot. Not suitable for use in ELISA or other applications where native protein is required. Recommended Dilutions: Western Blot: 10ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels. Researchers should empirically determine the suitability of the Granulocyte Colony Stimulating Factor, Recombinant, Human, Western Blot Positive Control for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Granulocyte Colony Stimulating Factor, Recombinant, Human, Western Blot Positive Control, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
