Human Golgi pH regulator B ELISA Kit | GPR89B elisa kit
Human Golgi pH regulator B (GPR89B) ELISA Kit
Gene Names
GPR89A; GPHR; GPR89; SH120; GPR89B; UNQ192
Reactivity
Human
Synonyms
Golgi pH regulator B; Human Golgi pH regulator B (GPR89B) ELISA Kit; GPR89B elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human GPR89B. No significant cross-reactivity or interference between Human GPR89B and analogues was observed.
Sequence Length
455
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for GPR89B elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GPR89B in samples. An antibody specific for GPR89B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGPR89B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GPR89B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPR89B bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
52,917 Da
NCBI Official Full Name
Golgi pH regulator A isoform 1
NCBI Official Synonym Full Names
G protein-coupled receptor 89A
NCBI Official Symbol
GPR89A
NCBI Official Synonym Symbols
GPHR; GPR89; SH120; GPR89B; UNQ192
NCBI Protein Information
Golgi pH regulator A
UniProt Protein Name
Golgi pH regulator B
Protein Family
UniProt Gene Name
GPR89B
UniProt Synonym Gene Names
GPHRB; GPR89C
NCBI Description
GPR89A is a nearly identical copy of the GPR89B gene (MIM 612806).[supplied by OMIM, Jun 2009]
Uniprot Description
GPR89B: Voltage dependent anion channel required for acidification and functions of the Golgi apparatus that may function in counter-ion conductance. Belongs to the Golgi pH regulator (TC 1.A.38) family. Homotrimer
Protein type: Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 1q21.2
Cellular Component: integral component of membrane
Molecular Function: voltage-gated anion channel activity
Biological Process: intracellular pH reduction; ion transport; protein transport
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Product Notes
The Human GPR89B gpr89b (Catalog #AAA280434) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA280434 ELISA Kit recognizes Human GPR89B. It is sometimes possible for the material contained within the vial of "Golgi pH regulator B, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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