Canine G Protein Coupled Receptor 48 ELISA Kit | GPR48 elisa kit

Canine G Protein Coupled Receptor 48 ELISA Kit

Cat. Num. AAA745182

• Gene Names: LGR4; GPR48; BNMD17
• Reactivity: Canine
• Synonyms: G Protein Coupled Receptor 48; Canine G Protein Coupled Receptor 48 ELISA Kit; GPR48 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This GRP48 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine GRP48. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GPR48 elisa kit

Principle of the assay: GRP48 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GRP48 antibody and an GRP48-HRP conjugate. The assay sample and buffer are incubated together with GRP48-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GRP48 concentration since GRP48 from samples and GRP48-HRP conjugate compete for the anti-GRP48 antibody binding site. Since the number of sites is limited, as more sites are occupied by GRP48 from the sample, fewer sites are left to bind GRP48-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GRP48 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for GPR48 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 7739737
• NCBI GeneID: 55366
• Molecular Weight: 101,667 Da
• NCBI Official Full Name: G-protein-coupled receptor 48
• NCBI Official Synonym Full Names: leucine-rich repeat containing G protein-coupled receptor 4
• NCBI Official Symbol: LGR4
• NCBI Official Synonym Symbols: GPR48; BNMD17
• NCBI Protein Information: leucine-rich repeat-containing G-protein coupled receptor 4; G protein-coupled receptor 48
• UniProt Protein Name: Leucine-rich repeat-containing G-protein coupled receptor 4
• UniProt Gene Name: LGR4
• UniProt Synonym Gene Names: GPR48
• UniProt Entry Name: LGR4_HUMAN

NCBI Description

G protein-coupled receptors (GPCRs) play key roles in a variety of physiologic functions. Members of the leucine-rich GPCR (LGR) family, such as GPR48, have multiple N-terminal leucine-rich repeats (LRRs) and a 7-transmembrane domain (Weng et al., 2008 [PubMed 18424556]).[supplied by OMIM, Aug 2008]

Uniprot Description

LGR4: a leucine-rich repeat-containing G-protein-coupled receptor. Receptor for R-spondins, potent stimulators of adult stem cell proliferation. A Wnt receptor component that enhances Wnt signaling by soluble R-spondin proteins. Plays an essential role during homeostasis of the adult mouse intestine.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1; Receptor, GPCR

Chromosomal Location of Human Ortholog: 11p14-p13

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; protein binding; transmembrane receptor activity

Biological Process: positive regulation of transcription, DNA-dependent; negative regulation of cytokine secretion; bone remodeling; bone mineralization; osteoblast differentiation; G-protein coupled receptor protein signaling pathway; epithelial cell proliferation; negative regulation of toll-like receptor signaling pathway; male genitalia development; innate immune response; spermatogenesis; circadian regulation of gene expression; negative regulation of transcription, DNA-dependent

Disease: Bone Mineral Density Quantitative Trait Locus 17